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PMID: 1018229 Published · ppublish English Journal Article

Manganese-dependent propagated action potentials and their depression by electrical stimulation in guinea-pig myocardium perfused by sodium-free media.

The Journal of physiology ·Vol. 263 ·No. 2 ·1976-12-00 ·Pages 139-56

Ochi R

Abstract

1. Propagated action potentials were recorded in right ventricular papillary muscles from guinea-pig heart while exposed to Na-free, Ca-free and Mg-free solutions containing Mn. 2. When Na was totally replaced by 95 mM-Mn the overshoot was about 45 mV while the resting potential was about -90mV. 3. The overshoot of action potentials was increased by about 20-30 mV per tenfold increase of Mn concentration over the range of 2-50 mM. 4. Similar increases of overshoots with increasing of Mn concentration also occurred in the presence of 0-6 mM-Ca. Increasing of Ca from 5 to 20 mM had little influence on the overshoot but shortened the duration of the Mn-dependent action potential in the presence of 5 mM-MN. 5 Mn-dependent action potentials were not depressed by 3 X 10(5) M tetrodotoxin but by La. 6. These results suggest that Mn passes through the slow inward current channel to generate the action potential seen under the Na-free condition. 7. The overshoot and duration of the Mn-dependent action potential decreased with stimulation. At stimulus frequencies (Hz) of 0-5, 0-2, 0-1, 0-017 and 0-0033 the overshoot of action potential in 5 mM-Mn Tyrode decreased by 0-5-1 mV per an action potential. This depression of the action potential is explained by assuming intracellular accumulation of Mn.

MeSH Terms
Action Potentials/drug effects Animals Calcium/pharmacology Guinea Pigs In Vitro Techniques Lanthanum/pharmacology Manganese/pharmacology Membrane Potentials/drug effects Papillary Muscles/drug effects,physiology Tetrodotoxin/pharmacology
Chemicals
Manganese Tetrodotoxin Lanthanum Calcium
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Ochi R
References (39)
39 references, click to expand
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1976.sp011625
Published
1976-12-00
Pages
139-56
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1307694
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1976.sp011625 PMC Full Text View journal details
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