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PMID: 10193766 Published · ppublish English Journal Article

Prostanoid receptors involved in the relaxation of human bronchial preparations.

British journal of pharmacology ·Vol. 126 ·No. 4 ·1999-02-00 ·Pages 867-72

Norel X, Walch L, Labat C, Gascard JP, Dulmet E, Brink C

Abstract

1. Iloprost and cicaprost (IP-receptor agonists) induced relaxations in the histamine- (50 microM) contracted human bronchial preparations (pD2 values, 6.63+/-0.12 and 6.86+/-0.08; Emax values, 90+/-04 and 65+/-08% of the papaverine response for iloprost (n=6) and cicaprost (n=3), respectively). 2. Prostaglandin E2 (PGE2) and misoprostol (EP-receptor agonist) relaxed the histamine-contracted human bronchial preparations (pD2 values, 7.13+/-0.07 and 6.33+/-0.28; Emax values, 67+/-04 and 57+/-08% of the papaverine response for PGE2 (n=14) and misoprostol (n=4), respectively). In addition, both relaxations were inhibited by AH6809 (DP/EP1/EP2-receptor antagonist; 3 microM; n=5-6). 3. The PGE2-induced relaxations of human bronchial preparations were not modified by treatment with AH23848B (TP/EP4-receptor antagonist; 30 microM; n=4). 4. The contracted human bronchial preparations were significantly relaxed by prostaglandin D2 (PGD2) or by BW245C a DP-receptor agonist. However, these responses did not exceed 40% of the relaxation induced by papaverine. In addition, the relaxations induced by PGD2 were significantly inhibited by treatment with a DP-receptor antagonist BWA868C (0.1 microM; n=3). 5. These data suggest that the relaxation of human isolated bronchial preparations induced by prostanoids involved IP-, EP2- and to a lesser extent DP-receptors but not EP4-receptor.

MeSH Terms
Aged Bronchi/physiology Female Humans Hydantoins/pharmacology Iloprost/pharmacology In Vitro Techniques Male Middle Aged Muscle Relaxation Prostaglandin D2/pharmacology Prostaglandins E/pharmacology Receptors, Prostaglandin/physiology
Chemicals
Hydantoins Prostaglandins E Receptors, Prostaglandin BW 245C Iloprost Prostaglandin D2
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Norel X
CNRS ERS 566, Centre Chirurgical Marie Lannelongue, Le Plessis-Robinson, France. [email protected]
Walch L
Labat C
Gascard J P
Dulmet E
Brink C
References (31)
31 references, click to expand
  1. Characterization of prostanoid relaxant/inhibitory receptors (psi) using a highly selective agonist, TR4979.
    Br J Pharmacol. 1986 Jan;87(1):45-56 PMID: 3006854
  2. Different responsiveness of prostaglandin D2-sensitive systems to prostaglandin D2 and its analogues.
    Br J Pharmacol. 1985 Jun;85(2):367-75 PMID: 2992661
  3. (5Z)-carbacyclin discriminates between prostacyclin-receptors coupled to adenylate cyclase in vascular smooth muscle and platelets.
    Br J Pharmacol. 1987 Jan;90(1):255-61 PMID: 3545351
  4. Relaxation of isolated human pulmonary muscle preparations with prostacyclin (PGI2) and its analogs.
    Prostaglandins. 1987 Jun;33(6):845-54 PMID: 2445003
  5. The action of prostanoid receptor agonists and antagonists on smooth muscle and platelets.
    Br J Pharmacol. 1988 Jun;94(2):591-601 PMID: 3134971
  6. AH6809, a prostaglandin DP-receptor blocking drug on human platelets.
    Br J Pharmacol. 1988 Jul;94(3):745-54 PMID: 2460179
  7. The classification of prostaglandin DP-receptors in platelets and vasculature using BW A868C, a novel, selective and potent competitive antagonist.
    Br J Pharmacol. 1989 Feb;96(2):291-300 PMID: 2924081
  8. Functional and ligand binding studies suggest heterogeneity of platelet prostacyclin receptors.
    Br J Pharmacol. 1989 Jul;97(3):657-68 PMID: 2474350
  9. Characterization of the prostanoid receptor profile of enprostil and isomers in smooth muscle and platelets in vitro.
    Br J Pharmacol. 1989 Dec;98(4):1335-43 PMID: 2514950
  10. Octimibate, a potent non-prostanoid inhibitor of platelet aggregation, acts via the prostacyclin receptor.
    Br J Pharmacol. 1991 Jan;102(1):251-9 PMID: 1710526
  11. Inhibitory effects of BAY u3405 on prostanoid-induced contractions in human isolated bronchial and pulmonary arterial muscle preparations.
    Br J Pharmacol. 1991 Nov;104(3):591-5 PMID: 1797322
  12. The effect of oral prostaglandin E1 on airway responsiveness in asthmatic subjects.
    Pulm Pharmacol. 1989;2(3):121-4 PMID: 2520494
  13. Concurrent down-regulation of IP prostanoid receptors and the alpha-subunit of the stimulatory guanine-nucleotide-binding protein (Gs) during prolonged exposure of neuroblastoma x glioma cells to prostanoid agonists. Quantification and functional implications.
    Biochem J. 1992 Jul 15;285 ( Pt 2):529-36 PMID: 1379045
  14. Prostanoid-induced relaxation of precontracted cat ciliary muscle is mediated by EP2 and DP receptors.
    Invest Ophthalmol Vis Sci. 1992 Oct;33(11):3195-201 PMID: 1328113
  15. Prostaglandin receptors coupled to adenylyl cyclase in the iris-ciliary body of rabbits, cats and cows.
    Exp Eye Res. 1993 Mar;56(3):327-33 PMID: 8386102
  16. Effect of inhaled prostaglandin E2 on allergen-induced asthma.
    Am Rev Respir Dis. 1993 Jul;148(1):87-90 PMID: 8317820
  17. Characterization of the prostaglandin E2 sensitive (EP)-receptor in the rat isolated trachea.
    Br J Pharmacol. 1994 May;112(1):133-6 PMID: 8032634
  18. Characterization of dilator prostanoid receptors in the fetal rabbit ductus arteriosus.
    J Pharmacol Exp Ther. 1994 Oct;271(1):390-6 PMID: 7965740
  19. Characterization of prostanoid receptors on rat neutrophils.
    Br J Pharmacol. 1994 Oct;113(2):581-7 PMID: 7834211
  20. A study of prostacyclin mimetics distinguishes neuronal from neutrophil IP receptors.
    Eur J Pharmacol. 1995 May 24;278(3):265-9 PMID: 7589166
  21. 6-Isopropoxy-9-oxoxanthene-2-carboxylic acid (AH 6809), a human EP2 receptor antagonist.
    Biochem Pharmacol. 1995 Nov 9;50(10):1731-3 PMID: 7503778
  22. A novel subtype of the prostacyclin receptor expressed in the central nervous system.
    J Biol Chem. 1996 Mar 8;271(10):5901-6 PMID: 8621463
  23. Prostanoid receptors involved in the relaxation of human pulmonary vessels.
    Br J Pharmacol. 1999 Feb;126(4):859-66 PMID: 10193765
  24. Clinical, functional and pathogenetic aspects of bronchial reactivity to prostaglandins F2alpha, E1, and E2.
    Adv Prostaglandin Thromboxane Res. 1976;1:461-75 PMID: 136876
  25. Specific receptors for prostaglandins in airways.
    Prostaglandins. 1980 Jun;19(6):819-41 PMID: 6247749
  26. Effect of prostacyclin on antigen induced immediate bronchoconstriction in asthmatic patients.
    Prostaglandins Med. 1979 Jul;3(1):39-45 PMID: 399527
  27. The antiplatelet and cardiovascular actions of a new carbacyclin derivative (ZK 36 374)--equipotent to PGI2 in vitro.
    Naunyn Schmiedebergs Arch Pharmacol. 1981 Jun;316(3):252-5 PMID: 6166877
  28. The binding of [3H]-prostacyclin to membranes of a neuronal somatic hybrid.
    Br J Pharmacol. 1981 Mar;72(3):435-41 PMID: 6266565
  29. Studies on the characterisation of prostanoid receptors: a proposed classification.
    Prostaglandins. 1982 Nov;24(5):667-89 PMID: 6131499
  30. Airway and cardiovascular responses to inhaled prostacyclin in normal and asthmatic subjects.
    Am Rev Respir Dis. 1985 Jan;131(1):18-21 PMID: 2981491
  31. Prostaglandin E receptor subtypes in smooth muscle: agonist activities of stable prostacyclin analogues.
    Br J Pharmacol. 1986 Jan;87(1):97-107 PMID: 2420404
Article Info
Journal
British journal of pharmacology
Abbr.
Br J Pharmacol
ISSN
0007-1188
DOI
10.1038/sj.bjp.0702392
Email
[email protected]
Published
1999-02-00
Pages
867-72
Language
English
Region
England
NLM ID
7502536
PMCID
PMC1571227
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.bjp.0702392 PMC Full Text View journal details
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