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PMID: 10199572 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nonsense mutations in the alcohol dehydrogenase gene of Drosophila melanogaster correlate with an abnormal 3' end processing of the corresponding pre-mRNA.

RNA (New York, N.Y.) ·Vol. 5 ·No. 4 ·1999-04-00 ·Pages 562-73

Brogna S

Abstract

From bacteria to mammals, mutations that generate premature termination codons have been shown to result in the reduction in the abundance of the corresponding mRNA. In mammalian cells, more often than not, the reduction happens while the RNA is still associated with the nucleus. Here, it is reported that mutations in the alcohol dehydrogenase gene (Adh) of Drosophila melanogaster that generate premature termination codons lead to reduced levels of cytoplasmic and nuclear mRNA. Unexpectedly, it has been found that the poly(A) tails of Adh mRNAs and pre-mRNAs that carry a premature termination codon are longer than in the wild-type transcript. The more 5' terminal the mutation is, the longer is the poly(A) tail of the transcript. These findings suggest that the integrity of the coding region may be required for accurate mRNA 3' end processing.

MeSH Terms
Alcohol Dehydrogenase/genetics Animals Base Sequence Codon, Terminator Drosophila melanogaster/enzymology,genetics Molecular Sequence Data Mutation Poly A/genetics RNA Precursors/genetics RNA Processing, Post-Transcriptional RNA Splicing/genetics RNA, Messenger/genetics Ribonuclease H/metabolism
Chemicals
Codon, Terminator RNA Precursors RNA, Messenger Poly A Alcohol Dehydrogenase Ribonuclease H
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Brogna S
University of Cambridge, Department of Genetics, Downing Site, United Kingdom. [email protected]
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
DOI
10.1017/s1355838299981359
Email
[email protected]
Published
1999-04-00
Pages
562-73
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1369782
Subset
IM
External Links
PubMed source DOI: 10.1017/s1355838299981359 PMC Full Text View journal details
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