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PMID: 1030703 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Purification from black widow spider venom of a protein factor causing the depletion of synaptic vesicles at neuromuscular junctions.

The Journal of cell biology ·Vol. 68 ·No. 3 ·1976-03-00 ·Pages 462-79

Frontali N, Ceccarelli B, Gorio A, Mauro A, Siekevitz P, Tzeng MC, Hurlbut WP

Abstract

The aqueous extract of the venom glands of black widow spiders was fractionated on a column of Sephadex G-200 and then on a column of DEAE-Sephadex A-50 pH 8.2. A protein fraction was obtained that caused a great increase in the frequency of occurrence of miniature end plate potentials at the frog neuromuscular junction, and caused swelling of the nerve terminals and depleted them of their vesicles. The fraction consists of a least four protein components that are similar in their molecular weights (about 130,000) and isoelectric points (ranging from pH 5.2 to 5.5) and are immunologically indistinguishable. It contains no sugar residues and has little or no lipolytic or proteolytic activity. The fraction is toxic to mice and is different from the fractions that act on houseflies, the crayfish stretch receptor and the cockroach heart. It seems pure enough to warrant a detailed study of its site and mode of action.

MeSH Terms
Animals Anura Astacoidea/drug effects Black Widow Spider Cockroaches/drug effects Heart/drug effects Isoelectric Point Mechanoreceptors/drug effects Membrane Potentials/drug effects Mice Molecular Weight Neuromuscular Junction/drug effects Proteins/analysis,isolation & purification,pharmacology,toxicity Spiders Synaptic Vesicles/drug effects Venoms/analysis,pharmacology
Chemicals
Proteins Venoms
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Frontali N
Ceccarelli B
Gorio A
Mauro A
Siekevitz P
Tzeng M C
Hurlbut W P
References (32)
32 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.68.3.462
Published
1976-03-00
Pages
462-79
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2109651
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.68.3.462 PMC Full Text View journal details
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