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PMID: 10319461 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov.

International journal of systematic bacteriology ·Vol. 49 Pt 2 ·1999-04-00 ·Pages 405-13

Coenye T, Falsen E, Vancanneyt M, Hoste B, Govan JR, Kersters K, Vandamme P

Abstract

A polyphasic taxonomic study that included DNA-DNA hybridizations, DNA base ratio determinations, 16S rDNA sequence analysis, whole-cell protein and fatty acid analyses, AFLP (amplified fragment length polymorphism) fingerprinting and an extensive biochemical characterization was performed on 10 strains provisionally identified as Alcaligenes faecalis-like bacteria. The six environmental and four human isolates belonged to the genus Ralstonia and were assigned to a new species for which the name Ralstonia gilardii sp. nov. is proposed. The type strain is LMG 5886T.

MeSH Terms
Bacterial Proteins/chemistry Bacterial Typing Techniques Base Composition DNA, Bacterial/chemistry,genetics DNA, Ribosomal/chemistry,genetics Electrophoresis, Polyacrylamide Gel Environmental Microbiology Fatty Acids/analysis Gram-Negative Aerobic Rods and Cocci/chemistry,classification,genetics,isolation & purification Gram-Negative Bacterial Infections/microbiology Humans Microbial Sensitivity Tests Molecular Sequence Data Nucleic Acid Hybridization RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA
Chemicals
Bacterial Proteins DNA, Bacterial DNA, Ribosomal Fatty Acids RNA, Ribosomal, 16S
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Coenye T
Laboratorium voor Microbiologie, Universiteit Gent, Belgium. [email protected]
Falsen E
Vancanneyt M
Hoste B
Govan J R
Kersters K
Vandamme P
Article Info
Journal
International journal of systematic bacteriology
Abbr.
Int J Syst Bacteriol
ISSN
0020-7713
Published
1999-04-00
Pages
405-13
Language
English
Region
England
NLM ID
0042143
Subset
IM
Databases
GENBANK
AF076645, AF085226
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