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PMID: 10329584 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

alpha-catenin-deficient F9 cells differentiate into signet ring cells.

The American journal of pathology ·Vol. 154 ·No. 5 ·1999-05-00 ·Pages 1323-8

Maeno Y, Moroi S, Nagashima H, Noda T, Shiozaki H, Monden M, Tsukita S, Nagafuchi A

Abstract

It has been demonstrated that alpha-catenin is frequently lost in diffuse type adenocarcinomas. We have isolated alpha-catenin-deficient mouse teratocarcinoma F9 cells by gene targeting. Wild-type F9 cell aggregates cultured in the presence of retinoic acid differentiated into embryoid bodies with an outer layer of epithelial cells. In contrast, cell aggregates of alpha-catenin-deficient cells did not develop outer layers under the same conditions. The outer surface cells of alpha-catenin-deficient cell aggregates, however, differentiated into epithelial cells as determined by their expression of epithelial marker proteins. These differentiated cells scattered from aggregates and showed signet ring cell morphology, which is frequently observed in diffuse type adenocarcinomas. We have provided clear evidence that a single mutation in the alpha-catenin gene may be a direct cause not only of the scattered properties of cells but also of signet ring cell formation in diffuse type adenocarcinoma.

MeSH Terms
Animals Cadherins/physiology Carcinoma, Signet Ring Cell/pathology,physiopathology Cell Differentiation/physiology Clone Cells/physiology Cytoskeletal Proteins/deficiency Epithelial Cells/physiology Mice Morphogenesis Tumor Cells, Cultured alpha Catenin
Chemicals
Cadherins Ctnna1 protein, mouse Cytoskeletal Proteins alpha Catenin
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Maeno Y
Department of Cell Biology, Faculty of Medicine, Kyoto University, Kyoto, Japan.
Moroi S
Nagashima H
Noda T
Shiozaki H
Monden M
Tsukita S
Nagafuchi A
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
DOI
10.1016/s0002-9440(10)65385-0
Published
1999-05-00
Pages
1323-8
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1866578
Subset
IM
External Links
PubMed source DOI: 10.1016/s0002-9440(10)65385-0 PMC Full Text View journal details
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