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PMID: 10330469 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Arabidopsis FUSCA5 encodes a novel phosphoprotein that is a component of the COP9 complex.

The Plant cell ·Vol. 11 ·No. 5 ·1999-05-00 ·Pages 839-48

Karniol B, Malec P, Chamovitz DA

Abstract

The COP9 complex is a regulator essential for repression of light-mediated development in Arabidopsis. Using partial amino acid sequence data generated from purified COP9 complexes, we cloned the Arabidopsis cDNA encoding the 27-kD subunit of the COP9 complex and showed that it is encoded by the previously identified FUSCA5 (FUS5) locus. fus5 mutants exhibit constitutive photomorphogenic phenotypes similar to those of cop9 and fus6. Point mutations in FUS5 that led to a loss of FUS5 protein were detected in four fus5 allelic strains. FUS5 contains the PCI/PINT and mitogen-activated protein kinase kinase activation loop motifs and is highly conserved with the mammalian COP9 complex subunit 7 and the Aspergillus nidulans AcoB proteins. FUS5 is present in both complex and monomeric forms. In the COP9 complex, FUS5 may interact directly with FUS6 and COP9. Mutations in FUS6 and COP9 result in a shift in the electrophoretic mobility of FUS5. This shift can be mimicked by in vitro phosphorylation of FUS5 by plant extracts. These findings further support the hypothesis that the COP9 complex is a central and common regulator that may interact with multiple signaling pathways.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics Base Sequence COP9 Signalosome Complex Genes, Plant Molecular Sequence Data Multiprotein Complexes Mutation Peptide Hydrolases Plant Proteins/genetics,metabolism Protein Binding Protein Conformation Proteins
Chemicals
Multiprotein Complexes Plant Proteins Proteins Peptide Hydrolases COP9 Signalosome Complex
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Karniol B
Department of Plant Sciences, Tel Aviv University, Tel Aviv 69978, Israel.
Malec P
Chamovitz D A
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.11.5.839
Published
1999-05-00
Pages
839-48
Language
English
Region
England
NLM ID
9208688
PMCID
PMC144222
Subset
IM
Databases
GENBANK
U89959
External Links
PubMed source DOI: 10.1105/tpc.11.5.839 PMC Full Text View journal details
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