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PMID: 10348851 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcriptional control of the iron-responsive fxbA gene by the mycobacterial regulator IdeR.

Journal of bacteriology ·Vol. 181 ·No. 11 ·1999-06-00 ·Pages 3402-8

Dussurget O, Timm J, Gomez M, Gold B, Yu S, Sabol SZ, Holmes RK, Jacobs WR, Smith I

Abstract

Exochelin is the primary extracellular siderophore of Mycobacterium smegmatis, and the iron-regulated fxbA gene encodes a putative formyltransferase, an essential enzyme in the exochelin biosynthetic pathway (E. H. Fiss, Y. Yu, and W. R. Jacobs, Jr., Mol. Microbiol. 14:557-569, 1994). We investigated the regulation of fxbA by the mycobacterial IdeR, a homolog of the Corynebacterium diphtheriae iron regulator DtxR (M. P. Schmitt, M. Predich, L. Doukhan, I. Smith, and R. K. Holmes, Infect. Immun. 63:4284-4289, 1995). Gel mobility shift experiments showed that IdeR binds to the fxbA regulatory region in the presence of divalent metals. DNase I footprinting assays indicated that IdeR binding protects a 28-bp region containing a palindromic sequence of the fxbA promoter that was identified in primer extension assays. fxbA regulation was measured in M. smegmatis wild-type and ideR mutant strains containing fxbA promoter-lacZ fusions. These experiments confirmed that fxbA expression is negatively regulated by iron and showed that inactivation of ideR results in iron-independent expression of fxbA. However, the levels of its expression in the ideR mutant were approximately 50% lower than those in the wild-type strain under iron limitation, indicating an undefined positive role of IdeR in the regulation of fxbA.

MeSH Terms
Bacterial Proteins/genetics,metabolism Base Sequence Binding Sites Cations, Divalent/pharmacology DNA Footprinting DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation, Bacterial/drug effects Genes, Bacterial/genetics Genes, Reporter Hydroxymethyl and Formyl Transferases/genetics Iron/pharmacology Mutation Mycobacterium/drug effects,enzymology,genetics Promoter Regions, Genetic/genetics Protein Binding/drug effects RNA, Messenger/analysis,genetics Repressor Proteins Response Elements/genetics Sequence Homology, Amino Acid Transcription, Genetic/drug effects,genetics beta-Galactosidase/genetics,metabolism
Chemicals
Bacterial Proteins Cations, Divalent DNA-Binding Proteins IdeR protein, Mycobacterium tuberculosis RNA, Messenger Repressor Proteins Iron Hydroxymethyl and Formyl Transferases beta-Galactosidase
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Dussurget O
TB Center, Public Health Research Institute, New York, New York 10016, USA.
Timm J
Gomez M
Gold B
Yu S
Sabol S Z
Holmes R K
Jacobs W R
Smith I
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.11.3402-3408.1999
Published
1999-06-00
Pages
3402-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC93806
Subset
IM
Grants
NIAID NIH HHS · R37 AI026170 · United States
NIAID NIH HHS · AI-26170 · United States
NIAID NIH HHS · R37 AI014107 · United States
NIGMS NIH HHS · GM 32651 · United States
NIAID NIH HHS · AI-14107 · United States
NIAID NIH HHS · R01 AI026170 · United States
NIAID NIH HHS · R01 AI014107 · United States
External Links
PubMed source DOI: 10.1128/JB.181.11.3402-3408.1999 PMC Full Text View journal details
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