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PMID: 1035580 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genetic characterization of methotrexate-resistant chinese hamster ovary cells.

In vitro ·Vol. 12 ·No. 11 ·1976-11-00 ·Pages 749-57

Flintoff WF, Spindler SM, Siminovitch L

Abstract

In a previous report, we described the selection and partial characterization of three methotrexate (Mtx)-resistant Chinese hamster ovary cells (CHO) (1). Class I cells contained an apparent structural alteration in dihydrofolate reductase. Class II cells had an alteration affecting the permeability of the drug. Class III cells, selected from Class I cells, had an increased activity of the altered enzyme. In the work described here, it has been shown that the spontaneous mutation rate to Class I resistance is in the order of 2 X 10-9 mutations per locus per generation and that in single-step mutagenized selections the number of resistant colonies of Class I and II are about equal. Class I and Class III resistance is expressed codominantly in somatic cell hybrids, whereas the Class II resistant marker is a recessive trait.

MeSH Terms
Animals Cell Line Cells, Cultured/drug effects Cricetinae Drug Resistance Female Hybrid Cells/enzymology Hybridization, Genetic Methotrexate/pharmacology Mutation Ovary Phenotype Tetrahydrofolate Dehydrogenase/metabolism
Chemicals
Tetrahydrofolate Dehydrogenase Methotrexate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Flintoff W F
Spindler S M
Siminovitch L
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21 references, click to expand
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Article Info
Journal
In vitro
Abbr.
In Vitro
ISSN
0073-5655
DOI
10.1007/BF02835450
Published
1976-11-00
Pages
749-57
Language
English
Region
United States
NLM ID
0063733
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02835450
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