Home LiteratureArticle Details
PMID: 10368162 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Biochemical and molecular characterization of the Bacillus subtilis acetoin catabolic pathway.

Journal of bacteriology ·Vol. 181 ·No. 12 ·1999-06-00 ·Pages 3837-41

Huang M, Oppermann-Sanio FB, Steinbüchel A

Abstract

A recent study indicated that Bacillus subtilis catabolizes acetoin by enzymes encoded by the acu gene cluster (F. J. Grundy, D. A. Waters, T. Y. Takova, and T. M. Henkin, Mol. Microbiol. 10:259-271, 1993) that are completely different from those in the multicomponent acetoin dehydrogenase enzyme system (AoDH ES) encoded by aco gene clusters found before in all other bacteria capable of utilizing acetoin as the sole carbon source for growth. By hybridization with a DNA probe covering acoA and acoB of the AoDH ES from Clostridium magnum, genomic fragments from B. subtilis harboring acoA, acoB, acoC, acoL, and acoR homologous genes were identified, and some of them were functionally expressed in E. coli. Furthermore, acoA was inactivated in B. subtilis by disruptive mutagenesis; these mutants were impaired to express PPi-dependent AoDH E1 activity to remove acetoin from the medium and to grow with acetoin as the carbon source. Therefore, acetoin is catabolized in B. subtilis by the same mechanism as all other bacteria investigated so far, leaving the function of the previously described acu genes obscure.

MeSH Terms
Acetoin/metabolism Acetoin Dehydrogenase/genetics,metabolism Bacillus subtilis/genetics,metabolism Chromosomes, Bacterial Escherichia coli/genetics Gene Library Kinetics Multigene Family Mutation Open Reading Frames Restriction Mapping
Chemicals
Acetoin Acetoin Dehydrogenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Huang M
Institut für Mikrobiologie der Westfälischen Wilhelms-Universität Münster, D-48149 Münster, Germany.
Oppermann-Sanio F B
Steinbüchel A
References (31)
31 references, click to expand
  1. Molecular characterization of the Pseudomonas putida 2,3-butanediol catabolic pathway.
    FEMS Microbiol Lett. 1994 Dec 1;124(2):141-50 PMID: 7813883
  2. Regulation of the Bacillus subtilis alsS, alsD, and alsR genes involved in post-exponential-phase production of acetoin.
    J Bacteriol. 1993 Jun;175(12):3863-75 PMID: 7685336
  3. The -24/-12 promoter comes of age.
    FEMS Microbiol Rev. 1989 Dec;5(4):341-57 PMID: 2517036
  4. Biochemical and molecular characterization of the Clostridium magnum acetoin dehydrogenase enzyme system.
    J Bacteriol. 1994 Jun;176(12):3614-30 PMID: 8206840
  5. Sequence analysis, biogenesis, and mitochondrial import of the alpha-subunit of rat liver propionyl-CoA carboxylase.
    J Biol Chem. 1989 Jul 25;264(21):12680-5 PMID: 2745462
  6. Multiple procaryotic ribonucleic acid polymerase sigma factors.
    Microbiol Rev. 1986 Sep;50(3):227-43 PMID: 3095618
  7. A common structural motif in thiamin pyrophosphate-binding enzymes.
    FEBS Lett. 1989 Sep 11;255(1):77-82 PMID: 2792374
  8. Histone deacetylases, acetoin utilization proteins and acetylpolyamine amidohydrolases are members of an ancient protein superfamily.
    Nucleic Acids Res. 1997 Sep 15;25(18):3693-7 PMID: 9278492
  9. Identification of genes involved in utilization of acetate and acetoin in Bacillus subtilis.
    Mol Microbiol. 1993 Oct;10(2):259-71 PMID: 7934817
  10. Cloning and sequencing of a 40.6 kb segment in the 73 degrees-76 degrees region of the Bacillus subtilis chromosome containing genes for trehalose metabolism and acetoin utilization.
    Microbiology. 1996 Nov;142 ( Pt 11):3057-65 PMID: 8969503
  11. Sequence conservation in the alpha and beta subunits of pyruvate dehydrogenase and its similarity to branched-chain alpha-keto acid dehydrogenase.
    FEBS Lett. 1991 Apr 22;282(1):209-13 PMID: 2026261
  12. Ultraviolet inactivation and excision-repair in Bacillus subtilis. I. Construction and characterization of a transformable eightfold auxotrophic strain and two ultraviolet-sensitive derivatives.
    Mutat Res. 1972 May;15(1):1-10 PMID: 4623569
  13. Catabolic repression of bacterial sporulation.
    Proc Natl Acad Sci U S A. 1965 Sep;54(3):704-11 PMID: 4956288
  14. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  15. THE BACTERIAL DESTRUCTION OF ACETYL-METHYL-CARBINOL.
    J Bacteriol. 1928 Jul;16(1):43-8 PMID: 16559317
  16. Identification and molecular characterization of the Alcaligenes eutrophus H16 aco operon genes involved in acetoin catabolism.
    J Bacteriol. 1991 Jul;173(13):4056-71 PMID: 2061286
  17. Purification and characterization of acetoin:2,6-dichlorophenolindophenol oxidoreductase, dihydrolipoamide dehydrogenase, and dihydrolipoamide acetyltransferase of the Pelobacter carbinolicus acetoin dehydrogenase enzyme system.
    J Bacteriol. 1991 Jan;173(2):757-67 PMID: 1898934
  18. Fast recovery of DNA from agarose gels by centrifugation through blotting paper.
    Trends Genet. 1991 Apr;7(4):109 PMID: 2068778
  19. The complete genome sequence of the gram-positive bacterium Bacillus subtilis.
    Nature. 1997 Nov 20;390(6657):249-56 PMID: 9384377
  20. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants.
    Proc Natl Acad Sci U S A. 1990 Jun;87(12):4645-9 PMID: 2162051
  21. Bacillus subtilis can modulate its capacity and specificity for protein secretion through temporally controlled expression of the sipS gene for signal peptidase I.
    Mol Microbiol. 1996 Nov;22(4):605-18 PMID: 8951809
  22. Multiple in vivo roles for the -12-region elements of sigma 54 promoters.
    J Bacteriol. 1998 Nov;180(21):5626-31 PMID: 9791111
  23. Compilation and analysis of Escherichia coli promoter DNA sequences.
    Nucleic Acids Res. 1983 Apr 25;11(8):2237-55 PMID: 6344016
  24. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  25. Dihydrolipoamide dehydrogenase: functional similarities and divergent evolution of the pyridine nucleotide-disulfide oxidoreductases.
    Arch Biochem Biophys. 1989 Feb 1;268(2):409-25 PMID: 2643922
  26. Characterization of anaerobic fermentative growth of Bacillus subtilis: identification of fermentation end products and genes required for growth.
    J Bacteriol. 1997 Nov;179(21):6749-55 PMID: 9352926
  27. Identification and molecular characterization of the aco genes encoding the Pelobacter carbinolicus acetoin dehydrogenase enzyme system.
    J Bacteriol. 1994 Jan;176(2):469-85 PMID: 8110297
  28. A multifunctional fermentative alcohol dehydrogenase from the strict aerobe Alcaligenes eutrophus: purification and properties.
    Eur J Biochem. 1984 Jun 15;141(3):555-64 PMID: 6378632
  29. Catabolite regulation of Bacillus subtilis acetate and acetoin utilization genes by CcpA.
    J Bacteriol. 1994 Aug;176(15):4527-33 PMID: 7913927
  30. [Relations between catabolite repression and sporulation in Bacillus subtilis (author's transl)].
    Arch Microbiol. 1976 Aug;109(1-2):181-6 PMID: 822795
  31. Acetoin catabolic system of Klebsiella pneumoniae CG43: sequence, expression, and organization of the aco operon.
    J Bacteriol. 1994 Jun;176(12):3527-35 PMID: 8206829
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.12.3837-3841.1999
Published
1999-06-00
Pages
3837-41
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC93865
Subset
IM
Databases
GENBANK
AF006075
External Links
PubMed source DOI: 10.1128/JB.181.12.3837-3841.1999 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2