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PMID: 10368955 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Networks and groups within the genus Neisseria: analysis of argF, recA, rho, and 16S rRNA sequences from human Neisseria species.

Molecular biology and evolution ·Vol. 16 ·No. 6 ·1999-06-00 ·Pages 773-83

Smith NH, Holmes EC, Donovan GM, Carpenter GA, Spratt BG

Abstract

To understand the pattern of nucleotide sequence variation among bacteria that frequently exchange chromosomal genes, we analyzed sequences of the recA, argF, and rho genes, as well as part of the small-subunit (16S) rRNA gene, from about 50 isolates of human commensal Neisseria species and the pathogenic N. meningitidis and N. gonorrhoeae. Almost all isolates of these species could be assigned to five phylogenetic groups that are found for all genes examined and generally are supported by high bootstrap values. In contrast, the phylogenetic relationships among groups varied according to the gene analyzed with notable incongruences involving N. cinerea and N. lactamica. Further analysis using split decomposition showed that for each gene, including 16S rRNA, the patterns of sequence divergence within N. meningitidis and closely related species were inconsistent with a bifurcating treelike phylogeny and better represented by an interconnected network. These data indicate that the human commensal Neisseria species can be separated into discrete groups of related species but that the relationships both within and among these groups, including those reconstructed using 16S rRNA, have been distorted by interspecies recombination events.

MeSH Terms

Base Sequence DNA Primers/genetics Evolution, Molecular Genes, Bacterial Humans Molecular Sequence Data Nasopharynx/microbiology Neisseria/classification,genetics,pathogenicity Neisseria gonorrhoeae/genetics Neisseria meningitidis/genetics Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Rec A Recombinases/genetics Rho Factor/genetics Sequence Homology, Nucleic Acid Species Specificity

Chemicals

DNA Primers RNA, Bacterial RNA, Ribosomal, 16S Rho Factor Rec A Recombinases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Smith N H

School of Biological Sciences, University of Sussex, Brighton, U.K.

Holmes E C

Donovan G M

Carpenter G A

Spratt B G

Article Info

Journal

Molecular biology and evolution

Abbr.

Mol Biol Evol

ISSN

0737-4038

DOI

10.1093/oxfordjournals.molbev.a026162

Published

1999-06-00

Pages

773-83

Language

English

Region

United States

NLM ID

8501455

Subset

Grants

Wellcome Trust · United Kingdom

Databases

GENBANK

AJ239278, AJ239279, AJ239280, AJ239281, AJ239282, AJ239283, AJ239284, AJ239285, AJ239286, AJ239287, AJ239288, AJ239289, AJ239290, AJ239291, AJ239292, AJ239293, AJ239294, AJ239295, AJ239296, AJ239297, AJ239298, AJ239299, AJ239300, AJ239301, AJ239302, AJ239303, AJ239304, AJ239305, AJ239306, AJ239307

External Links

PubMed source DOI: 10.1093/oxfordjournals.molbev.a026162 View journal details

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Networks and groups within the genus Neisseria: analysis of argF, recA, rho, and 16S rRNA sequences from human Neisseria species.

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