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PMID: 10375634 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of expressed sequence tags of porcine skeletal muscle.

Gene ·Vol. 233 ·No. 1-2 ·1999-06-11 ·Pages 181-8

Davoli R, Zambonelli P, Bigi D, Fontanesi L, Russo V

Abstract

Porcine skeletal muscle genes play a major role in determining muscle growth and meat quality. Therefore, to progress towards a better understanding of the genetic factors influencing these traits, the first step is to characterize the genes expressed in skeletal muscle tissue in pig. To this aim, we constructed a porcine biceps femoris muscle cDNA library and sequenced 111 randomly isolated clones. By FASTA analysis, we identified 72 unique clones: 47 showed homology to previously identified genes in human or other mammals, 20 matched uncharacterized expressed sequence tags (ESTs), two showed no significant matches to sequences already present in DNA databases, and three other clones containing only repetitive elements were excluded from further analysis. Mitochondrial genes (16.2%), myosin heavy chain genes (9%) and the actin alpha skeletal muscle gene (9%) were the most abundant transcripts. Among the 47 identified genes several muscle-specific or predominant sequences expressed in skeletal muscle were found. The sequences of the clones matching uncharacterized human, mouse or porcine ESTs were tested by GRAIL in order to identify putative coding regions. The results of our analysis allowed the establishment of a first list of genes expressed in porcine skeletal muscle.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA, Complementary Expressed Sequence Tags Molecular Sequence Data Muscle, Skeletal/metabolism Open Reading Frames RNA, Messenger/genetics Swine

Chemicals

DNA, Complementary RNA, Messenger

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Davoli R

DIPROVAL, Sezione di Allevamenti Zootecnici, University of Bologna, Via F.lli Rosselli 107, Coviolo, 42100 Reggio Emilia, Italy. [email protected]

Zambonelli P

Bigi D

Fontanesi L

Russo V

Article Info

Journal

Gene

Abbr.

Gene

ISSN

0378-1119

DOI

10.1016/s0378-1119(99)00141-9

[email protected]

Published

1999-06-11

Pages

181-8

Language

English

Region

Netherlands

NLM ID

7706761

Subset

Databases

GENBANK

X91845, X91846, X91847, X91849, X91850, X94252, X94253, X94254, X94689, Y18404, Y18405, Z98764, Z98765, Z98766, Z98767, Z98768, Z98769, Z98770, Z98771, Z98773, Z98774, Z98775, Z98776, Z98777, Z98778, Z98779, Z98780, Z98781, Z98782, Z98783

External Links

PubMed source DOI: 10.1016/s0378-1119(99)00141-9 View journal details

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Analysis of expressed sequence tags of porcine skeletal muscle.

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