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PMID: 10383953 Published · ppublish English Journal Article

Amino acid residues in the omega-minus region participate in cellular localization of yeast glycosylphosphatidylinositol-attached proteins.

Journal of bacteriology ·Vol. 181 ·No. 13 ·1999-07-00 ·Pages 3886-9

Hamada K, Terashima H, Arisawa M, Yabuki N, Kitada K

Abstract

The final destination of glycosylphosphatidylinositol (GPI)-attached proteins in Saccharomyces cerevisiae is the plasma membrane or the cell wall. Two kinds of signals have been proposed for their cellular localization: (i) the specific amino acid residues V, I, or L at the site 4 or 5 amino acids upstream of the GPI attachment site (the omega site) and Y or N at the site 2 amino acids upstream of the omega site for cell wall localization and (ii) dibasic residues in the region upstream of the omega site (the omega-minus region) for plasma membrane localization. The relationships between these amino acid residues and efficiencies of cell wall incorporation were examined by constructing fusion reporter proteins from open reading frames encoding putative GPI-attached proteins. The levels of incorporation were high in the constructs containing the specific amino acid residues and quite low in those containing two basic amino acid residues in the omega-minus region. With constructs that contained neither specific residues nor two basic residues, levels of incorporation were moderate. These correlations clearly suggest that GPI-attached proteins have two different signals which act positively or negatively in cell wall incorporation for their cellular localization.

MeSH Terms
Amino Acid Sequence Biological Transport Cell Compartmentation/physiology Cell Membrane/metabolism Cell Wall/metabolism Genes, Reporter Glycoproteins/metabolism Glycosylphosphatidylinositols/metabolism Molecular Sequence Data Saccharomyces cerevisiae/metabolism
Chemicals
Glycoproteins Glycosylphosphatidylinositols
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hamada K
Department of Mycology, Nippon Roche Research Center, Kamakura, Kanagawa 247-8530, Japan.
Terashima H
Arisawa M
Yabuki N
Kitada K
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.13.3886-3889.1999
Published
1999-07-00
Pages
3886-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC93875
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.181.13.3886-3889.1999 PMC Full Text View journal details
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