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PMID: 10397769 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

UNC-11, a Caenorhabditis elegans AP180 homologue, regulates the size and protein composition of synaptic vesicles.

Molecular biology of the cell ·Vol. 10 ·No. 7 ·1999-07-00 ·Pages 2343-60

Nonet ML, Holgado AM, Brewer F, Serpe CJ, Norbeck BA, Holleran J, Wei L, Hartwieg E, Jorgensen EM, Alfonso A

Abstract

The unc-11 gene of Caenorhabditis elegans encodes multiple isoforms of a protein homologous to the mammalian brain-specific clathrin-adaptor protein AP180. The UNC-11 protein is expressed at high levels in the nervous system and at lower levels in other tissues. In neurons, UNC-11 is enriched at presynaptic terminals but is also present in cell bodies. unc-11 mutants are defective in two aspects of synaptic vesicle biogenesis. First, the SNARE protein synaptobrevin is mislocalized, no longer being exclusively localized to synaptic vesicles. The reduction of synaptobrevin at synaptic vesicles is the probable cause of the reduced neurotransmitter release observed in these mutants. Second, unc-11 mutants accumulate large vesicles at synapses. We propose that the UNC-11 protein mediates two functions during synaptic vesicle biogenesis: it recruits synaptobrevin to synaptic vesicle membranes and it regulates the size of the budded vesicle during clathrin coat assembly.

MeSH Terms
Adaptor Proteins, Vesicular Transport Amino Acid Sequence Animals Caenorhabditis elegans/genetics,metabolism Caenorhabditis elegans Proteins Clathrin/biosynthesis Endocytosis Helminth Proteins/genetics,metabolism Homozygote Intracellular Membranes/metabolism Membrane Proteins/genetics,metabolism Molecular Sequence Data Monomeric Clathrin Assembly Proteins Mutation Nerve Tissue Proteins/genetics,metabolism Nervous System/metabolism Neurotransmitter Agents/metabolism Phosphoproteins/genetics Protein Isoforms R-SNARE Proteins Sequence Homology, Amino Acid Synaptic Vesicles/metabolism,ultrastructure Vertebrates
Chemicals
Adaptor Proteins, Vesicular Transport Caenorhabditis elegans Proteins Clathrin Helminth Proteins Membrane Proteins Monomeric Clathrin Assembly Proteins Nerve Tissue Proteins Neurotransmitter Agents Phosphoproteins Protein Isoforms R-SNARE Proteins clathrin assembly protein AP180 unc-11 protein, C elegans
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Nonet M L
Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA. [email protected]
Holgado A M
Brewer F
Serpe C J
Norbeck B A
Holleran J
Wei L
Hartwieg E
Jorgensen E M
Alfonso A
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.10.7.2343
Email
[email protected]
Published
1999-07-00
Pages
2343-60
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC25452
Subset
IM
Grants
NIGMS NIH HHS · R01 GM024663 · United States
NIGMS NIH HHS · GM-24663 · United States
NINDS NIH HHS · R37 NS034307 · United States
NINDS NIH HHS · R01 NS034307 · United States
NIGMS NIH HHS · R37 GM024663 · United States
NINDS NIH HHS · NS-34307 · United States
NINDS NIH HHS · R01 NS033535 · United States
NINDS NIH HHS · NS-32449 · United States
Databases
GENBANK
AF144257, AF144258, AF144259, AF144260, AF144261, AF144262
External Links
PubMed source DOI: 10.1091/mbc.10.7.2343 PMC Full Text View journal details
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