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PMID: 10417386 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An adhesin of the yeast pathogen Candida glabrata mediating adherence to human epithelial cells.

Science (New York, N.Y.) ·Vol. 285 ·No. 5427 ·1999-07-23 ·Pages 578-82

Cormack BP, Ghori N, Falkow S

Abstract

Candida glabrata is an important fungal pathogen of humans that is responsible for about 15 percent of mucosal and systemic candidiasis. Candida glabrata adhered avidly to human epithelial cells in culture. By means of a genetic approach and a strategy allowing parallel screening of mutants, it was possible to clone a lectin from a Candida species. Deletion of this adhesin reduced adherence of C. glabrata to human epithelial cells by 95 percent. The adhesin, encoded by the EPA1 gene, is likely a glucan-cross-linked cell-wall protein and binds to host-cell carbohydrate, specifically recognizing asialo-lactosyl-containing carbohydrates.

MeSH Terms
Amino Acid Sequence Animals Calcium/metabolism Candida/genetics,pathogenicity,physiology Candidiasis, Vulvovaginal/microbiology Carbohydrates/pharmacology Cell Adhesion Cloning, Molecular Epithelial Cells/microbiology Female Fungal Proteins Genes, Fungal Humans Lectins/chemistry,genetics,metabolism Ligands Mice Mice, Inbred DBA Molecular Sequence Data Mutagenesis, Insertional Mutation Plasmids Polymerase Chain Reaction Transformation, Genetic Tumor Cells, Cultured Virulence/genetics
Chemicals
Carbohydrates Fungal Proteins Lectins Ligands epithelial adhesin 1, Candida glabrata Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cormack B P
Department of Microbiology and Immunology, Stanford University School of Medicine, Fairchild D039, 299 Campus Drive, Stanford, CA 94305-5124, USA. [email protected]
Ghori N
Falkow S
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.285.5427.578
Email
[email protected]
Published
1999-07-23
Pages
578-82
Language
English
Region
United States
NLM ID
0404511
Subset
IM
Corrections
CommentIn
PMID: 10447486
External Links
PubMed source DOI: 10.1126/science.285.5427.578 View journal details
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