Molecular characterization of genetic loci required for secretion of exoproducts in Burkholderia pseudomallei.

Journal of bacteriology ·Vol. 181 ·No. 15 ·1999-08-00 ·Pages 4661-4

DeShazer D, Brett PJ, Burtnick MN, Woods DE

Abstract

Previous studies have demonstrated that Burkholderia pseudomallei secretes protease, lipase, and phospholipase C (PLC) into the extracellular milieu, but their mechanisms of secretion and roles in pathogenesis have not been elucidated. In this study, we isolated and characterized 29 transposon mutants unable to secrete protease, lipase, and PLC.

MeSH Terms

Burkholderia pseudomallei/enzymology,genetics Chromosome Mapping Chromosomes, Bacterial Endopeptidases/biosynthesis,genetics Escherichia coli/genetics Genes, Bacterial Lipase/biosynthesis,genetics Molecular Sequence Data Multigene Family Mutagenesis, Insertional Plasmids Type C Phospholipases/biosynthesis,genetics

Chemicals

Lipase Type C Phospholipases Endopeptidases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

DeShazer D

Genetics and Physiology Branch, Bacteriology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702, USA.

Brett P J

Burtnick M N

Woods D E

References (11)

11 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/JB.181.15.4661-4664.1999

Published

1999-08-00

Pages

4661-4

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC103600

Subset

Databases

GENBANK

AF110185, AF110186

External Links

PubMed source DOI: 10.1128/JB.181.15.4661-4664.1999 PMC Full Text View journal details

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Molecular characterization of genetic loci required for secretion of exoproducts in Burkholderia pseudomallei.

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