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PMID: 10436024 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Syntaxin is required for cell division.

Molecular biology of the cell ·Vol. 10 ·No. 8 ·1999-08-00 ·Pages 2735-43

Conner SD, Wessel GM

Abstract

We recently identified a single family member homologue of syntaxin in the sea urchin. Syntaxin is present throughout development, and in rapidly dividing cleavage stage embryos it is present on numerous vesicles at the cell cortex. We hypothesized that syntaxin mediates essential membrane fusion events during early embryogenesis, reasoning that the vesicles and/or their contents are important for development. Here we show that functional inactivation of syntaxin with either Botulinum neurotoxin C1, which specifically proteolyzes syntaxin, or antibodies against syntaxin results in an inhibition of cell division. These observations suggest that syntaxin is essential for membrane fusion events critical for cell division.

MeSH Terms
Animals Antibodies/pharmacology Botulinum Toxins/pharmacology Cell Division/drug effects,physiology Cell Membrane/physiology Dose-Response Relationship, Drug Embryo, Nonmammalian/cytology,metabolism Membrane Fusion/physiology Membrane Proteins/drug effects,immunology,metabolism Qa-SNARE Proteins Sea Urchins/drug effects,embryology
Chemicals
Antibodies Membrane Proteins Qa-SNARE Proteins Botulinum Toxins botulinum toxin type C
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Conner S D
Department of Molecular and Cellular Biology & Biochemistry, Brown University, Providence, Rhode Island 02912, USA.
Wessel G M
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.10.8.2735
Published
1999-08-00
Pages
2735-43
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC25508
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.10.8.2735 PMC Full Text View journal details
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