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PMID: 10456883 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Intracellular growth in Acanthamoeba castellanii affects monocyte entry mechanisms and enhances virulence of Legionella pneumophila.

Infection and immunity ·Vol. 67 ·No. 9 ·1999-09-00 ·Pages 4427-34

Cirillo JD, Cirillo SL, Yan L, Bermudez LE, Falkow S, Tompkins LS

Abstract

Since Legionella pneumophila is an intracellular pathogen, entry into and replication within host cells are thought to be critical to its ability to cause disease. L. pneumophila grown in one of its environmental hosts, Acanthamoeba castellanii, is phenotypically different from L. pneumophila grown on standard laboratory medium (BCYE agar). Although amoeba-grown L. pneumophila displays enhanced entry into monocytes compared to BCYE-grown bacteria, the mechanisms of entry used and the effects on virulence have not been examined. To explore whether amoeba-grown L. pneumophila differs from BCYE-grown L. pneumophila in these characteristics, we examined entry into monocytes, replication in activated macrophages, and virulence in mice. Entry of amoeba-grown L. pneumophila into monocytes occurred more frequently by coiling phagocytosis, was less affected by complement opsonization, and was less sensitive to microtubule and microfilament inhibitors than was entry of BCYE-grown bacteria. In addition, amoeba-grown L. pneumophila displays increased replication in monocytes and is more virulent in A/J, C57BL/6 Beige, and C57BL/6 mice. These data demonstrate for the first time that the intra-amoebal growth environment affects the entry mechanisms and virulence of L. pneumophila.

MeSH Terms
Acanthamoeba/microbiology Animals Cell Line Colchicine/pharmacology Complement System Proteins/immunology Cytochalasin D/pharmacology Humans Intracellular Fluid Legionella pneumophila/growth & development,immunology,pathogenicity Mice Mice, Inbred C57BL Monocytes/microbiology Nocodazole/pharmacology Phagocytosis Virulence
Chemicals
Cytochalasin D Complement System Proteins Nocodazole Colchicine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cirillo J D
Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln, Lincoln, Nebraska 68583, USA. [email protected]
Cirillo S L
Yan L
Bermudez L E
Falkow S
Tompkins L S
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.67.9.4427-4434.1999
Email
[email protected]
Published
1999-09-00
Pages
4427-34
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC96761
Subset
IM
Grants
NIAID NIH HHS · T32 AI007328 · United States
NIAID NIH HHS · AI07328 · United States
NIAID NIH HHS · AI30618 · United States
NIAID NIH HHS · AI40165 · United States
External Links
PubMed source DOI: 10.1128/IAI.67.9.4427-4434.1999 PMC Full Text View journal details
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