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PMID: 10464188 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Distinct types of rRNA operons exist in the genome of the actinomycete Thermomonospora chromogena and evidence for horizontal transfer of an entire rRNA operon.

Journal of bacteriology ·Vol. 181 ·No. 17 ·1999-09-00 ·Pages 5201-9

Yap WH, Zhang Z, Wang Y

Abstract

We describe here the presence of two distinct types of rRNA operons in the genome of a thermophilic actinomycete Thermomonospora chromogena. The genome of T. chromogena contains six rRNA operons (rrn), of which four complete and two incomplete ones were cloned and sequenced. Comparative analysis revealed that the operon rrnB exhibits high levels of sequence variations to the other five nearly identical ones throughout the entire length of the operon. The coding sequences for the 16S and 23S rRNA genes differ by approximately 6 and 10%, respectively, between the two types of operons. Normal functionality of rrnB is concluded on the basis of the nonrandom distribution of nucleotide substitutions, the presence of compensating nucleotide covariations, the preservation of secondary and tertiary rRNA structures, and the detection of correctly processed rRNAs in the cell. Comparative sequence analysis also revealed a close evolutionary relationship between rrnB operon of T. chromogena and rrnA operon of another thermophilic actinomycete Thermobispora bispora. We propose that T. chromogena acquired rrnB operon from T. bispora or a related organism via horizontal gene transfer.

MeSH Terms

Actinomycetales/classification,genetics Base Sequence Blotting, Southern Cloning, Molecular DNA, Bacterial DNA, Ribosomal/analysis Evolution, Molecular Gene Amplification Gene Expression Genetic Variation Genome, Bacterial Molecular Sequence Data Nucleic Acid Conformation Polymerase Chain Reaction RNA, Bacterial RNA, Ribosomal, 16S Sequence Analysis, DNA rRNA Operon

Chemicals

DNA, Bacterial DNA, Ribosomal RNA, Bacterial RNA, Ribosomal, 16S

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Yap W H

Microbial Collection and Screening Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, Singapore 117609.

Zhang Z

Wang Y

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/JB.181.17.5201-5209.1999

Published

1999-09-00

Pages

5201-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC94023

Subset

Databases

GENBANK

AF116216, AF116217, AF116218, AF116219, AF116220, AF116221, AF116222, AF116223, AF116224, AF116225, AF116226, AF116227, AF116228, AF116229, AF116230, AF116231, AF116232, AF116233, AF116234, AF116235, AF116236, AF116558, AF116559, AF116560, AF116561, AF116562, AF116563

External Links

PubMed source DOI: 10.1128/JB.181.17.5201-5209.1999 PMC Full Text View journal details

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Distinct types of rRNA operons exist in the genome of the actinomycete Thermomonospora chromogena and evidence for horizontal transfer of an entire rRNA operon.

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