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PMID: 10481917 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Structure of the alpha-actinin rod: molecular basis for cross-linking of actin filaments.

Cell ·Vol. 98 ·No. 4 ·1999-08-20 ·Pages 537-46

Djinović-Carugo K, Young P, Gautel M, Saraste M

Abstract

We have determined the crystal structure of the two central repeats in the alpha-actinin rod at 2.5 A resolution. The repeats are connected by a helical linker and form a symmetric, antiparallel dimer in which the repeats are aligned rather than staggered. Using this structure, which reveals the structural principle that governs the architecture of alpha-actinin, we have devised a plausible model of the entire alpha-actinin rod. The electrostatic properties explain how the two alpha-actinin subunits assemble in an antiparallel fashion, placing the actin-binding sites at both ends of the rod. This molecular architecture results in a protein that is able to form cross-links between actin filaments.

MeSH Terms
Actin Cytoskeleton/chemistry Actins/chemistry Amino Acid Sequence Connectin Crystallography, X-Ray Cytoskeletal Proteins Dimerization Glycoproteins Humans Macromolecular Substances Metalloproteins/chemistry Models, Molecular Molecular Sequence Data Muscle Proteins/chemistry Protein Conformation Protein Kinases/chemistry Protein Structure, Secondary Recombinant Fusion Proteins/chemistry Repetitive Sequences, Amino Acid Sequence Alignment Spectrin/chemistry Static Electricity Structure-Activity Relationship Talin/chemistry Vinculin/chemistry Zyxin
Chemicals
Actins Connectin Cytoskeletal Proteins Glycoproteins Macromolecular Substances Metalloproteins Muscle Proteins Recombinant Fusion Proteins TTN protein, human Talin ZYX protein, human Zyxin Vinculin Spectrin Protein Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Djinović-Carugo K
Structural Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
Young P
Gautel M
Saraste M
Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/s0092-8674(00)81981-9
Published
1999-08-20
Pages
537-46
Language
English
Region
United States
NLM ID
0413066
Subset
IM
Databases
PDB
1QUU
External Links
PubMed source DOI: 10.1016/s0092-8674(00)81981-9 View journal details
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