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PMID: 10500226 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Regeneration of a germinal layer in the adult mammalian brain.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 20 ·1999-09-28 ·Pages 11619-24

Doetsch F, García-Verdugo JM, Alvarez-Buylla A

Abstract

Neuronal precursors reside in the subventricular zone (SVZ) of adult mammals. This region is composed of a network of chains of migrating neuroblasts ensheathed by astrocytes and juxtaposed by clusters of immature precursors (type C cells). Here we show that after antimitotic treatment with cytosine-beta-D-arabinofuranoside, neuroblasts and type C cells are eliminated but some astrocytes remain. Remarkably, the SVZ network rapidly regenerates. Soon after cytosine-beta-D-arabinofuranoside treatment astrocytes divide. Two days later, type C cells reappear, followed at 4.5 days by migrating neuroblasts. By 10 days the SVZ network is fully regenerated, and the orientation and organization of chains of migrating neuroblasts resemble that of normal mice. This regeneration reveals an unexpected plasticity in the adult central nervous system and should provide a model system to study the early stages of neurogenesis in the adult brain.

MeSH Terms
Animals Brain/cytology,drug effects,physiology Cell Communication Cytarabine/pharmacology Glial Fibrillary Acidic Protein/analysis Immunohistochemistry Male Mice Microscopy, Electron Neural Cell Adhesion Molecules/analysis Regeneration Thymidine/metabolism
Chemicals
Glial Fibrillary Acidic Protein Neural Cell Adhesion Molecules Cytarabine Thymidine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Doetsch F
The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
García-Verdugo J M
Alvarez-Buylla A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.20.11619
Published
1999-09-28
Pages
11619-24
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC18083
Subset
IM
Grants
NICHD NIH HHS · R01 HD032116 · United States
NICHD NIH HHS · R37 HD032116 · United States
NICHD NIH HHS · HD32116 · United States
NINDS NIH HHS · NS28378 · United States
External Links
PubMed source DOI: 10.1073/pnas.96.20.11619 PMC Full Text View journal details
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