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PMID: 10515996 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of intracellular reverse transcription complexes of Moloney murine leukemia virus.

Journal of virology ·Vol. 73 ·No. 11 ·1999-11-00 ·Pages 8919-25

Fassati A, Goff SP

Abstract

To examine the early events in the life cycle of Moloney murine leukemia virus (MoMLV), we analyzed the intracellular complexes mediating reverse transcription. Partial purification of the reverse transcription complexes (RTCs) by equilibrium density fractionation and velocity sedimentation indicated that three distinct species of intracellular complexes are formed shortly after cell infection. Only one of these species is able to start and complete reverse transcription in the cell cytoplasm. This RTC is composed of at least the viral genome, capsid, integrase, and reverse transcriptase proteins. The RTC becomes permeable to micrococcal nuclease but not to antibodies. Shortly after initiation of reverse transcription, the viral strong stop DNA within the RTC is protected from nuclease digestion. The sedimentation velocity of the RTC decreases during reverse transcription. After entry into the nucleus, most capsid proteins are lost from the RTC and its sedimentation velocity decreases further.

MeSH Terms
Blotting, Western Capsid/analysis Centrifugation, Density Gradient Chemical Fractionation DNA, Viral/biosynthesis,metabolism Detergents Genome, Viral Integrases/analysis Moloney murine leukemia virus/chemistry,genetics,metabolism Polymerase Chain Reaction/methods RNA-Directed DNA Polymerase/analysis,metabolism Transcription, Genetic Virion Virus Integration
Chemicals
DNA, Viral Detergents Integrases RNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fassati A
Department of Biochemistry, Howard Hughes Medical Institute, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.
Goff S P
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.73.11.8919-8925.1999
Published
1999-11-00
Pages
8919-25
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC112922
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.73.11.8919-8925.1999 PMC Full Text View journal details
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