Specific labelling of replicating SPP1 DNA: analysis of viral DNA synthesis and identification of phage DNA-genes.

Molecular & general genetics : MGG ·Vol. 166 ·No. 3 ·1978-11-09 ·Pages 277-85

Burger KJ, Trautner TA

Abstract

Specific labelling of replicating bacteriophage SPP1 DNA can be achieved by infection at nonpermissive temperature of a B. subtilis strain carrying the initation mutation dnaB ts134. Under these conditions host DNA synthesis is reduced by 90 to 95%. This technique was used to identify cistrons of SPP1 involved in phage DNA synthesis and to define intermediates in SPP1 replication.

MeSH Terms

Bacillus subtilis Bacteriophages/genetics DNA Replication DNA, Viral/genetics Genes, Viral Genetic Techniques

Chemicals

DNA, Viral

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Burger K J

Trautner T A

References (23)

23 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

Published

1978-11-09

Pages

277-85

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source

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Specific labelling of replicating SPP1 DNA: analysis of viral DNA synthesis and identification of phage DNA-genes.

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