Regional preference of insertion of Tn501 and Tn802 into RP1 and its derivatives.

Molecular & general genetics : MGG ·Vol. 166 ·No. 3 ·1978-11-09 ·Pages 313-20

Grinsted J, Bennett PM, Higginson S, Richmond MH

Abstract

The sites of insertion of Tn501 into RP1 and into derivatives of this plasmid that either lack the Tn801 (TnA) element or contain it in a different location have been determined. Similarly, the sites of insertion of Tn802 into a derivative of RP1 that lacks the Tn801 element and into recombinants of this plasmid with Tn501 were determined. 'Hot spots' for insertion were observed with both transposons; but it is clear that a particular DNA sequence is not sufficient to define a 'hot spot', since a particular region does contain many insertions when present in one plasmid but does not do so when part of another.

MeSH Terms

Base Sequence Chromosomes, Bacterial DNA, Bacterial/genetics Drug Resistance, Microbial Escherichia coli/genetics Genes Pseudomonas aeruginosa/genetics R Factors Recombination, Genetic

Chemicals

DNA, Bacterial

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Grinsted J

Bennett P M

Higginson S

Richmond M H

References (24)

24 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00267624

Published

1978-11-09

Pages

313-20

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00267624

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Regional preference of insertion of Tn501 and Tn802 into RP1 and its derivatives.

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