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PMID: 10535943 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Ku is associated with the telomere in mammals.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 96 ·No. 22 ·1999-10-26 ·Pages 12454-8

Hsu HL, Gilley D, Blackburn EH, Chen DJ

Abstract

Telomeres are specialized DNA/protein complexes that comprise the ends of eukaryotic chromosomes. The highly expressed Ku heterodimer, composed of 70 and 80 K(d) subunits (Ku70 and Ku80), is the high-affinity DNA binding component of the DNA-dependent protein kinase. Ku is critical for nonhomologous DNA double-stranded break repair and site-specific recombination of V(D)J gene segments. Ku also plays an important role in telomere maintenance in yeast. Herein, we report, using an in vivo crosslinking method, that human and hamster telomeric DNAs specifically coimmunoprecipitate with human Ku80 after crosslinking. Localization of Ku to the telomere does not depend on the DNA-dependent protein kinase catalytic component. These findings suggest a direct link between Ku and the telomere in mammalian cells.

MeSH Terms
Animals Antigens, Nuclear CHO Cells Catalytic Domain Cricetinae DNA Helicases DNA-Activated Protein Kinase DNA-Binding Proteins/metabolism Dimerization Humans Ku Autoantigen Nuclear Proteins/metabolism Protein Serine-Threonine Kinases/metabolism Saccharomyces cerevisiae Proteins Telomere Tumor Cells, Cultured
Chemicals
Antigens, Nuclear DNA-Binding Proteins Nuclear Proteins Saccharomyces cerevisiae Proteins high affinity DNA-binding factor, S cerevisiae DNA-Activated Protein Kinase PRKDC protein, human Protein Serine-Threonine Kinases DNA Helicases XRCC5 protein, human Xrcc6 protein, human Ku Autoantigen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hsu H L
Life Sciences Division, Los Alamos National Laboratory, Los Alamos, NM 87545, USA.
Gilley D
Blackburn E H
Chen D J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.96.22.12454
Published
1999-10-26
Pages
12454-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC22947
Subset
IM
Grants
NIGMS NIH HHS · GM26259 · United States
NCI NIH HHS · R37 CA050519 · United States
NIGMS NIH HHS · R01 GM026259 · United States
NCI NIH HHS · CA50519 · United States
NIGMS NIH HHS · R37 GM026259 · United States
NCI NIH HHS · R01 CA050519 · United States
External Links
PubMed source DOI: 10.1073/pnas.96.22.12454 PMC Full Text View journal details
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