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PMID: 10542180 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The CpxRA signal transduction system of Escherichia coli: growth-related autoactivation and control of unanticipated target operons.

Journal of bacteriology ·Vol. 181 ·No. 21 ·1999-11-00 ·Pages 6772-8

De Wulf P, Kwon O, Lin EC

Abstract

In Escherichia coli, the CpxRA two-component signal transduction system senses and responds to aggregated and misfolded proteins in the bacterial envelope. We show that CpxR-P (the phosphorylated form of the cognate response regulator) activates cpxRA expression in conjunction with RpoS, suggesting an involvement of the Cpx system in stationary-phase survival. Engagement of the CpxRA system in functions beyond protein management is indicated by several putative targets identified after a genomic screening for the CpxR-P recognition consensus sequence. Direct negative control of the newly identified targets motABcheAW (specifying motility and chemotaxis) and tsr (encoding the serine chemoreceptor) by CpxR-P was shown by electrophoretic mobility shift analysis and Northern hybridization. The results suggest that the CpxRA system plays a core role in an extensive stress response network in which the coordination of protein turnover and energy conservation may be the unifying element.

MeSH Terms
Bacterial Proteins/genetics,metabolism Base Sequence Escherichia coli/genetics,growth & development,metabolism Escherichia coli Proteins Gene Expression Regulation, Bacterial Membrane Proteins/genetics,metabolism Methyl-Accepting Chemotaxis Proteins Molecular Sequence Data Operon Promoter Regions, Genetic Protein Kinases/genetics,metabolism Sigma Factor/genetics,metabolism Signal Transduction
Chemicals
Bacterial Proteins CheW protein, E coli Escherichia coli Proteins Membrane Proteins Methyl-Accepting Chemotaxis Proteins MotA protein, Bacteria MotB protein, Bacteria Sigma Factor Tsr protein, Bacteria sigma factor KatF protein, Bacteria tsr protein, E coli CheW protein, Bacteria CpxR protein, Bacteria Protein Kinases CpxA protein, E coli CpxA protein, bacteria
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
De Wulf P
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
Kwon O
Lin E C
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.181.21.6772-6778.1999
Published
1999-11-00
Pages
6772-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC94143
Subset
IM
Grants
NIGMS NIH HHS · GM39693 · United States
NIGMS NIH HHS · GM40993 · United States
External Links
PubMed source DOI: 10.1128/JB.181.21.6772-6778.1999 PMC Full Text View journal details
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