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PMID: 1054835 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Effects of cytochaslasin B and colcemide on myogenic cultures.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 2 ·1975-02-00 ·Pages 513-7

Holtzer H, Croop J, Dienstman S, Ishikawa H, Somlyo AP

Abstract

Muscle cultures treated with cytochalasin B yield mono- and oligonucleated cells of two kinds: (i) arborized, replicating precursor myogenic cells and fibroblasts; and (ii) round, post-mitotic, terminally differentiating myoblasts and myotubes. The arborized cells do not bind fluorescein-labeled antibody against myosin, do not contract rhythmically, and do not display hexagonally stacked thick and thin filaments. The round, mono-nucleated myoblasts and round, oligonucleated myotubes bind the fluorescein-labeled antibody against myosin, contract rhythmically, and display clusters of hexagonally-stacked thick and thin filaments. When cytochalasin B is removed and replaced by colcemide, the arborized cells, but not the post-mitotic muscle cells, acquire a radial symmetry and are induced to assemble massive, meandering cables that may occupy over 25% of the cell volume. These tortuous calbes are positively birefringent and consist exclusively of enormous numbers of 100-A, intermediate-sized filaments.

MeSH Terms
Animals Cell Differentiation/drug effects Cell Division/drug effects Cell Nucleus/ultrastructure Chick Embryo Colchicine/pharmacology Culture Techniques Cytochalasin B/pharmacology Muscles/cytology,drug effects,ultrastructure Myofibrils/ultrastructure
Chemicals
Cytochalasin B Colchicine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Holtzer H
Croop J
Dienstman S
Ishikawa H
Somlyo A P
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.2.513
Published
1975-02-00
Pages
513-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432342
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.2.513 PMC Full Text View journal details
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