Abstract
Somatic cell hybrids were generated by fusion of mouse erythroleukemia cells either to mouse L cells (B82), human fibroblasts (W1-18 VA2), or human marrow fractions enriched in erythroblasts. The hybrid cells were examined for globin gene expression by benzidine staining to detect cytoplasmic hemoglobin, and by molecular hybridization of cellular RNA to globin complementary DNA (cDNA) to detect globin messenger RNA (MRNA). The fibroblast (human or mouse) times erythroleukemia cell hybrids grown in monolayer retained most of the chromosomes of each parent. Neither cytoplasmic hemoglobin nor globin mRNA was detected in dimethylsulfoxide-treated fibroblast times erythroleukemia hybrid cells, indicating extinction of hemoglobin synthesis prior to the formation of cytoplasmic mRNA. The human marrow times mouse erythroleukemia hybrid cells grown in suspension culture contained only a few human chromosomes and exhibited low levels of hemoglobin synthesis which were amplified by 2% dimethylsulfoxide. Mouse (but not human) globin mRNA was demonstrated in these hybrid cells. The results suggest that somatic cell hybrids may be useful in searching for genetic factors which regulate activity of the globin genes.
MeSH Terms
Bone Marrow Cells
Cell Fusion
Cell Line
DNA/metabolism
Dimethyl Sulfoxide/pharmacology
Fibroblasts
Genes
Globins/biosynthesis
Hemoglobins/biosynthesis
Hybrid Cells/metabolism
L Cells
Leukemia, Erythroblastic, Acute
Nucleic Acid Hybridization
RNA, Messenger/biosynthesis,metabolism
Chemicals
Hemoglobins
RNA, Messenger
Globins
DNA
Dimethyl Sulfoxide
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Deisseroth A
Burk R
Picciano D
Anderson W F
Nienhuis A
Minna J
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