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PMID: 10565123 Published · ppublish jpn Comparative Study English Abstract Journal Article

[Use of a rapid detection assay for influenza virus, on nasal aspirate specimens].

Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases ·Vol. 73 ·No. 10 ·1999-10-00 ·Pages 1064-8

Yamazaki M, Kimura K, Watanabe S, Komiyama O, Mishiku Y, Yamamoto K, Sugaya N, Hashimoto Y, Hagiwara N, Maezawa T, Imai M

Abstract

We investigated the usefulness of a rapid antigen detection kit using optical immunoassay for influenza virus (FLU OIA, BioStar, USA). Nasal aspirates were taken from 92 influenza suspected outpatients between March to April of 1999. Compared with virus isolation and PCR, the sensitivity of FLU OIA was 88.5% and 81.6%, and the specificity was 65.2% and 72.2%. All isolated viruses were influenza type B virus. It was difficult to differentiate the weak-positive and negative cases, leading to the rather low specificity, although the assay procedure was easy and quick. FLU OIA may be a useful rapid diagnosis kit for influenza in pediatric outpatient clinics and wards, because it can detect both influenza type A and type B viruses.

MeSH Terms
Antigens, Viral/analysis Humans Immunoassay/methods Influenza B virus/immunology,isolation & purification Influenza, Human/diagnosis Mucus/virology Nasal Mucosa/virology Polymerase Chain Reaction Reagent Kits, Diagnostic/standards Sensitivity and Specificity
Chemicals
Antigens, Viral Reagent Kits, Diagnostic
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Yamazaki M
Department of Pediatrics, Isehara Kyodo Hospital.
Kimura K
Watanabe S
Komiyama O
Mishiku Y
Yamamoto K
Sugaya N
Hashimoto Y
Hagiwara N
Maezawa T
Imai M
Article Info
Journal
Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases
Abbr.
Kansenshogaku Zasshi
ISSN
0387-5911
Published
1999-10-00
Pages
1064-8
Language
jpn
Region
Japan
NLM ID
0236671
Subset
IM
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