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PMID: 10571227 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Goalpha and diacylglycerol kinase negatively regulate the Gqalpha pathway in C. elegans.

Neuron ·Vol. 24 ·No. 2 ·1999-10-00 ·Pages 323-33

Miller KG, Emerson MD, Rand JB

Abstract

We investigated the EGL-30 (Gqalpha) pathway in C. elegans by using genetic screens to identify genes that confer phenotypes similar to egl-30 mutants. One such gene, egl-8, encodes a phospholipase Cbeta that is present throughout the nervous system and near intestinal cell junctions. EGL-30 and EGL-8 appear to positively regulate synaptic transmission because reducing their function results in strong aldicarb resistance and slow locomotion rates. In contrast, GOA-1 (Goalpha) and DGK-1 (diacylglycerol kinase) appear to negatively regulate synaptic transmission, because reducing their function results in strong aldicarb hypersensitivity and hyperactive locomotion. A genetic analysis suggests that GOA-1 negatively regulates the EGL-30 pathway and that DGK-1 antagonizes the EGL-30 pathway.

MeSH Terms
Animals Caenorhabditis elegans/genetics,metabolism,physiology Diacylglycerol Kinase/physiology GTP-Binding Protein alpha Subunits, Gi-Go Helminth Proteins/genetics,physiology Heterotrimeric GTP-Binding Proteins/metabolism,physiology Intestinal Mucosa/metabolism Intestines/cytology Isoenzymes/genetics Molecular Sequence Data Mutation/physiology Nervous System/cytology,metabolism Phenotype Phospholipase C beta Synaptic Transmission/physiology Type C Phospholipases/genetics
Chemicals
Helminth Proteins Isoenzymes Diacylglycerol Kinase Type C Phospholipases Phospholipase C beta GTP-Binding Protein alpha Subunits, Gi-Go Heterotrimeric GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Miller K G
Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA.
Emerson M D
Rand J B
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Article Info
Journal
Neuron
Abbr.
Neuron
ISSN
0896-6273
DOI
10.1016/s0896-6273(00)80847-8
Published
1999-10-00
Pages
323-33
Language
English
Region
United States
NLM ID
8809320
PMCID
PMC4703424
Subset
IM
Grants
NINDS NIH HHS · F32 NS009565 · United States
NIGMS NIH HHS · R01 GM059642 · United States
NINDS NIH HHS · NS33187 · United States
Databases
GENBANK
AF179426
Corrections
CommentIn
PMID: 10571220
External Links
PubMed source DOI: 10.1016/s0896-6273(00)80847-8 PMC Full Text View journal details
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