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PMID: 1057168 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Visualization of actin fibers associated with the cell membrane in amoebae of Dictyostelium discoideum.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 5 ·1975-05-00 ·Pages 1758-62

Clarke M, Schatten G, Mazia D, Spudich JA

Abstract

Amoebae of Dictyostelium discoideum were attached to a surface coated with polylysine, and the upper portion of the cells was sheared off with a stream of buffer. Scanning and transmission electron microscopy showed that the cytoplasmic surface of the exposed membrane was covered with fibers consisting of actin-containing filaments. The actin was identified by its solubility properties and its ability to interact with muscle myosin.

MeSH Terms
Actins/analysis Amoeba/analysis,ultrastructure Binding Sites Cell Membrane/analysis,ultrastructure Dictyostelium/analysis,ultrastructure Klebsiella Microscopy, Electron Microscopy, Electron, Scanning Myxomycetes/analysis Polylysine Protein Binding
Chemicals
Actins Polylysine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Clarke M
Schatten G
Mazia D
Spudich J A
References (11)
11 references, click to expand
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    Proc Natl Acad Sci U S A. 1974 Nov;71(11):4561-4 PMID: 4612524
  9. Early contacts between fibroblasts. An ultrastructural study.
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.5.1758
Published
1975-05-00
Pages
1758-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432625
Subset
IM
Grants
NICHD NIH HHS · R37 HD012913 · United States
External Links
PubMed source DOI: 10.1073/pnas.72.5.1758 PMC Full Text View journal details
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