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PMID: 1057180 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Methylated, blocked 5 termini in HeLa cell mRNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 5 ·1975-05-00 ·Pages 1904-8

Furuichi Y, Morgan M, Shatkin AJ, Jelinek W, Salditt-Georgieff M, Darnell JE

Abstract

Poly(A)-containing HeLa cell mRNA prepared from cells labeled with [methyl-(3)H]methionine or [(32)P]phosphate was found to contain a variety of methylated, blocked 5'-terminal structures of two general types: m(7)GpppN(7)-Np and m(7)GpppN(m)-N(m)-Np. In addition, about one-third of the [(3)H]methyl label was present in the N(6)-methyladenosine; this labeled nucleoside was not found in the 3'-terminal one-third of the mRNA chain and thus may also be in the 5' portion of the mRNA.

MeSH Terms
Base Sequence Chromatography, DEAE-Cellulose HeLa Cells/analysis,metabolism Humans Methionine/metabolism Methylation Oligonucleotides/analysis Phosphates/metabolism RNA, Messenger/analysis,metabolism RNA, Neoplasm/analysis,metabolism Ribonucleases Ribonucleotides/analysis
Chemicals
Oligonucleotides Phosphates RNA, Messenger RNA, Neoplasm Ribonucleotides Methionine Ribonucleases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Furuichi Y
Morgan M
Shatkin A J
Jelinek W
Salditt-Georgieff M
Darnell J E
References (9)
9 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.5.1904
Published
1975-05-00
Pages
1904-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432656
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.5.1904 PMC Full Text View journal details
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