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PMID: 10583605 Published · ppublish English Journal Article

Immunomodulatory effect of a plasmid expressing CD40 ligand on DNA vaccination against human immunodeficiency virus type-1.

Immunology ·Vol. 98 ·No. 3 ·1999-11-00 ·Pages 436-42

Ihata A, Watabe S, Sasaki S, Shirai A, Fukushima J, Hamajima K, Inoue J, Okuda K

Abstract

CD40 ligand is a costimulatory molecule which acts a potent immunomodulator. We found the mice inoculated with human CD40 ligand expression plasmid (pMEhCD40L) combined with human immunodeficiency virus type-1 (HIV-1) DNA vaccine exhibited both humoral and cellular antigen-specific immunological enhancement. The expression of hCD40L induced predominantly antigen-specific immunoglobulin G (IgG) antibody response while it failed to induce mucosal IgA response. Delayed-type hypersensitivity (DTH) and cytotoxic T lymphocyte (CTL) activity were induced in a dose-dependent manner. Examination of the relative levels of the two IgG subclasses showed that co-injection of pMEhCD40L enhanced IgG2a response without suppressing IgG1 response. Similarly, the expression of pMEhCD40L enhanced not only T helper 1 (Th1)- but also Th2-type cytokine production. In conclusion, co-inoculation of pMEhCD40L with DNA vaccine was shown to be a useful way to enhance CTL responses without suppressing the humoral immune response in acquired immune deficiency syndrome (AIDS) patients.

MeSH Terms
AIDS Vaccines/administration & dosage Animals Antibody Formation/genetics CD40 Antigens CD40 Ligand Cytokines/metabolism Cytotoxicity Tests, Immunologic Dose-Response Relationship, Immunologic Enzyme-Linked Immunosorbent Assay HIV Infections/therapy HIV-1 Hypersensitivity, Delayed/immunology Immunity, Cellular/genetics Immunoglobulin G/metabolism Lymphocyte Activation Membrane Glycoproteins/administration & dosage,genetics Mice Mice, Inbred BALB C Plasmids/immunology T-Lymphocytes, Cytotoxic/immunology
Chemicals
AIDS Vaccines CD40 Antigens Cytokines Immunoglobulin G Membrane Glycoproteins CD40 Ligand
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ihata A
Department of Bacteriology, Yokohama City University School of Medicine, Japan.
Watabe S
Sasaki S
Shirai A
Fukushima J
Hamajima K
Inoue J
Okuda K
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
DOI
10.1046/j.1365-2567.1999.00879.x
Published
1999-11-00
Pages
436-42
Language
English
Region
England
NLM ID
0374672
PMCID
PMC2326951
Subset
IM
External Links
PubMed source DOI: 10.1046/j.1365-2567.1999.00879.x PMC Full Text View journal details
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