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PMID: 1058473 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Site-specific cleavage of single-stranded DNA by a Hemophilus restriction endonuclease.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 7 ·1975-07-00 ·Pages 2555-8

Horiuchi K, Zinder ND

Abstract

Single-stranded viral DNA of bacteriophage f1 is cleaved into specific fragments by endo R-HaeIII, a restriction endonuclease isolated from Hemophilus aegyptius. The sites of the single strand cleavage correspond to those of the double strand cleavage. A single-stranded DNA fragment containing only one HaeIII site is also cleaved by this enzyme. This observation suggests that the reaction of single-stranded DNA cleavage does not require the formation of a symmetrical double-stranded structure that would result from the intramolecular base-pairing between two different HaeIII sites. Other restriction endonucleases may also cleave single-stranded DNA.

MeSH Terms
Base Sequence Chromosome Mapping Coliphages DNA Restriction Enzymes/metabolism DNA Viruses DNA, Single-Stranded DNA, Viral Electrophoresis, Polyacrylamide Gel Endonucleases Haemophilus/enzymology Nucleic Acid Denaturation Nucleic Acid Renaturation
Chemicals
DNA, Single-Stranded DNA, Viral Endonucleases DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Horiuchi K
Zinder N D
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.7.2555
Published
1975-07-00
Pages
2555-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432807
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.7.2555 PMC Full Text View journal details
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