Home LiteratureArticle Details

PMID: 10587644 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

CUL-2 is required for the G1-to-S-phase transition and mitotic chromosome condensation in Caenorhabditis elegans.

Nature cell biology ·Vol. 1 ·No. 8 ·1999-12-00 ·Pages 486-92

Feng H, Zhong W, Punkosdy G, Gu S, Zhou L, Seabolt EK, Kipreos ET

Abstract

The human cullin protein CUL-2 functions in a ubiquitin-ligase complex with the von Hippel-Lindau (VHL) tumour suppressor protein. Here we show that, in Caenorhabditis elegans, cul-2 is expressed in proliferating cells and is required at two distinct points in the cell cycle, the G1-to-S-phase transition and mitosis. cul-2 mutant germ cells undergo a G1-phase arrest that correlates with accumulation of CKI-1, a member of the CIP/KIP family of cyclin-dependent-kinase inhibitors. In cul-2 mutant embryos, mitotic chromosomes are unable to condense, leading to unequal DNA segregation, chromosome bridging and the formation of multiple nuclei.

MeSH Terms

Amino Acid Sequence Animals Caenorhabditis elegans/cytology,embryology,genetics,metabolism Caenorhabditis elegans Proteins Cell Cycle Proteins/chemistry,genetics,metabolism Cell Lineage Cell Nucleus/genetics Chromosomes/chemistry,genetics,metabolism Cloning, Molecular Cullin Proteins Cyclin-Dependent Kinase Inhibitor Proteins Fluorescent Antibody Technique G1 Phase Gene Expression Regulation, Developmental Germ Cells/cytology,metabolism In Situ Hybridization Mitosis Molecular Conformation Molecular Sequence Data Mutation/genetics Nondisjunction, Genetic Phenotype RNA, Messenger/analysis,genetics S Phase

Chemicals

CUL2 protein, human Caenorhabditis elegans Proteins Cell Cycle Proteins Cullin 1 Cullin Proteins Cyclin-Dependent Kinase Inhibitor Proteins RNA, Messenger cki-1 protein, C elegans cki-2 protein, C elegans

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Feng H

Department of Cellular Biology, University of Georgia, Athens, Georgia 30602, USA.

Zhong W

Punkosdy G

Gu S

Zhou L

Seabolt E K

Kipreos E T

Article Info

Journal

Nature cell biology

Abbr.

Nat Cell Biol

ISSN

1465-7392

DOI

10.1038/70272

Published

1999-12-00

Pages

486-92

Language

English

Region

England

NLM ID

100890575

Subset

Grants

NIGMS NIH HHS · R01 GM55297 · United States

Databases

GENBANK

AF179358, AF179359

External Links

PubMed source DOI: 10.1038/70272 View journal details

Analysis Services

CUL-2 is required for the G1-to-S-phase transition and mitotic chromosome condensation in Caenorhabditis elegans.

Contact

WeChat Official Account