Home LiteratureArticle Details
PMID: 1059155 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mutants of T7 bacteriophage inhibited by lambda prophage.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 9 ·1975-09-00 ·Pages 3642-6

Pao CC, Speyer JF

Abstract

Mutants in gene 20, a new T7 gene, cannot grow on rex+ lambda lysogens. Gene 20-- mutants suppress in double mutants the phenotype of T7 ligase negative mutations, but not vice versa. Amber 20- mutants have been obtained. There are differences between these T7 mutations and the similar T4 rII mutations. There are host mutations which permit T7 20- mutants to grow on lambda+ lysogens. T7 DNA synthesis on normal lambda+ lysogens infected with 20- mutants is essentially normal, but the DNA is not packaged. The gene 20 protein is active in in vitro complementation and probably used late in infection for DNA packaging into phage heads.

MeSH Terms
Chromosome Mapping Coliphages/metabolism DNA Replication Lysogeny Mutation Virus Replication
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pao C C
Speyer J F
References (28)
28 references, click to expand
  1. Reversal of mutant phenotypes by 5-fluorouracil: an approach to nucleotide sequences in messenger-RNA.
    Proc Natl Acad Sci U S A. 1962 Apr 15;48:532-46 PMID: 13877975
  2. Physiological effects of rII mutations in bacteriophage T4.
    Virology. 1961 Jun;14:151-63 PMID: 13703644
  3. Lambda-repressed mutants of bacteriophage T5. I. Isolation and genetical characterization.
    Virology. 1973 Nov;56(1):230-7 PMID: 4583304
  4. Strains of phage lambda in current use.
    Virology. 1969 Jun;38(2):349-51 PMID: 4891412
  5. Genetics and function of DNA ligase in Escherichia coli.
    J Mol Biol. 1973 Jul 15;77(4):531-47 PMID: 4353284
  6. T7 translational control mechanisms and their inhibiton by F factors.
    Nat New Biol. 1971 May 12;231(19):37-41 PMID: 5283385
  7. Fast sedimenting bacteriophage T7 DNA from T7-infected Escherichia coli.
    Virology. 1974 May;59(1):70-88 PMID: 4274985
  8. Order of injection of T7 bacteriophage DNA.
    J Virol. 1973 Jun;11(6):1024-6 PMID: 4576515
  9. Reduction of colicin E2-induced DNA breakdown by the rex gene of lambda prophage.
    Virology. 1974 Jul;60(1):288-92 PMID: 4601462
  10. Bacteriophage T7.
    Science. 1972 Apr 28;176(4033):367-76 PMID: 4554613
  11. An endodeoxyribonuclease induced after infection with phage T7 bearing amber mutations in genes 3, 5, and 6.
    Can J Biochem. 1972 Sep;50(9):1016-23 PMID: 4561327
  12. Regulation of bacteriophage T5 development by ColI factors.
    J Virol. 1972 May;9(5):804-12 PMID: 4554465
  13. Bacteriophage T7-induced endonuclease II. Purification and properties of the enzyme.
    J Biol Chem. 1972 Jan 10;247(1):146-56 PMID: 4552921
  14. Packaging and maturation of DNA of bacteriophage T7 in vitro.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3545-9 PMID: 4530321
  15. Genetic analysis of non-essential bacteriophage T7 genes.
    J Mol Biol. 1973 Sep 15;79(2):227-36 PMID: 4760131
  16. A mutant of bacteriophage T7 deficient in polynucleotide ligase.
    J Biol Chem. 1971 Nov 25;246(22):6874-9 PMID: 5001611
  17. Partial suppression of bacteriophage T4 ligase mutations by T4 endonuclease II deficiency: role of host ligase.
    J Virol. 1971 Apr;7(4):534-6 PMID: 4939389
  18. The integration and excision defect of bacteriophage lambda-dg.
    J Mol Biol. 1969 Dec 28;46(3):565-80 PMID: 4904108
  19. On the role of ligase in genetic recombination in bacteriophage T4.
    Virology. 1969 Aug;38(4):701-3 PMID: 4896157
  20. T7-directed protein synthesis.
    Virology. 1969 Nov;39(3):575-86 PMID: 4902070
  21. The genetics and physiology of bacteriophage T7.
    Virology. 1969 Nov;39(3):562-74 PMID: 4902069
  22. DNA replication of phage T4 rII mutants without polynucleotide ligase (gene 30).
    Biochem Biophys Res Commun. 1969 Oct 22;37(3):416-22 PMID: 4900136
  23. Suppression of T4D ligase mutations by rIIa and rIIb mutations.
    Proc Natl Acad Sci U S A. 1969 Nov;64(3):897-904 PMID: 5264148
  24. An intermediate in the replication of bacteriophage T7 DNA molecules.
    J Mol Biol. 1969 Sep 28;44(3):459-75 PMID: 5346051
  25. The extent of rII deletions in phage T4.
    Genet Res. 1968 Apr;11(2):215-9 PMID: 5647634
  26. Phage lambda mutants deficient in r-II exclusion.
    Science. 1967 Dec 22;158(3808):1588-9 PMID: 6060364
  27. Restriction of the growth of bacteriophage BF23 by a colicine I (Col I-P9) factor.
    Virology. 1966 Apr;28(4):763-5 PMID: 5938892
  28. FINE STRUCTURE OF A GENETIC REGION IN BACTERIOPHAGE.
    Proc Natl Acad Sci U S A. 1955 Jun 15;41(6):344-54 PMID: 16589677
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.9.3642
Published
1975-09-00
Pages
3642-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433052
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.9.3642 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2