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PMID: 1060077 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Regulation of synthesis of hepatic fatty acid synthetase: binding of fatty acid synthetase antibodies to polysomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 10 ·1975-10-00 ·Pages 3956-60

Alberts AW, Strauss AW, Hennessy S, Vagelos PR

Abstract

Mammalian fatty acid synthetase was shown to be composed of two peptides, molecular weight 240,000, after dissociation with sodium dodecyl sulfate. Rat liver polysomes that synthesize fatty acid synthetase were identified by sucrose gradient analysis of polysomes that had been reacted with 125I-labeled antibody against fatty acid synthetase. The binding of 125I-labeled antibody to polysomes was found to correlate with the rate of hepatic fatty acid synthesis in various nutritional conditions.

MeSH Terms
Animals Antigen-Antibody Reactions Binding Sites, Antibody Cell Line Dietary Fats Fatty Acid Synthases/biosynthesis,immunology Liver/enzymology Macromolecular Substances Molecular Weight Polyribosomes/enzymology Rats Starvation
Chemicals
Dietary Fats Macromolecular Substances Fatty Acid Synthases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Alberts A W
Strauss A W
Hennessy S
Vagelos P R
References (23)
23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.10.3956
Published
1975-10-00
Pages
3956-60
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433116
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.10.3956 PMC Full Text View journal details
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