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PMID: 1060114 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Acetylcholine-receptor-mediated ion flux in electroplax membrane preparations.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 11 ·1975-11-00 ·Pages 4371-5

Hess GP, Andrews JP, Struve GE, Goombs SE

Abstract

The kinetics of acetylcholine-receptor-mediated sodium efflux from electroplax microsacs of electrophorus electricus has been analyzed. This led to the discovery that only a small fraction of the observed efflux is affected by chemical effectors such as carbamylcholine. Experimental conditions were chosen so that the receptor-mediated flux could be analyzed without the measurements' being obscured by efflux from the nonexcitable microsacs. Near equilibrium the efflux follows a single exponential decay. The apparent first order rate constant for sodium-22 efflux was determined as a function of effector concentration and is considerably higher than previously estimated. The process does not show cooperativity under the experimental conditions, in agreement with the binding isotherms of effectors and the same membrane preparation. The presence of potassium ions inhibits the receptor-mediated sodium flux. It is suggested that interaction of inorganic ions with the receptor may play an important role in the cooperative effects observed in electrophysiological experiments.

MeSH Terms
Acetylcholine Animals Binding Sites Biological Transport/drug effects Carbachol/metabolism Electric Organ/metabolism Electrophorus In Vitro Techniques Kinetics Potassium/pharmacology Receptors, Cholinergic Sodium/metabolism
Chemicals
Receptors, Cholinergic Carbachol Sodium Acetylcholine Potassium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hess G P
Andrews J P
Struve G E
Goombs S E
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.11.4371
Published
1975-11-00
Pages
4371-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388723
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.11.4371 PMC Full Text View journal details
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