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PMID: 1060117 Published · ppublish English Journal Article

Initiation of in vitro mRNA synthesis from the wild-type lac promoter.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 11 ·1975-11-00 ·Pages 4394-8

Majors J

Abstract

An in vitro transcription system, dependent on catabolite gene activator protein (CAP), utilizing a 200 base-pair restriction fragment, has been used to show that the initiation site of the wild-type Escherichia coli lac mRNA, and that of two mutants, 8d and ps, are identical to that previously reported for the CAP-independent promoter mutant UV5. Order of addition experiments are used to show that the binding of lac repressor to the operator is competitive with that of the RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) to the promoter, thus demonstrating functional overlap of the operator and promoter sites.

MeSH Terms
Base Sequence Binding Sites DNA-Directed RNA Polymerases/metabolism Genes, Regulator Lactose Operon RNA, Bacterial/biosynthesis RNA, Messenger/analysis,biosynthesis Transcription, Genetic
Chemicals
RNA, Bacterial RNA, Messenger DNA-Directed RNA Polymerases Lactose
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Majors J
References (27)
27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.11.4394
Published
1975-11-00
Pages
4394-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388728
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.11.4394 PMC Full Text View journal details
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