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PMID: 1060120 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Fusion of dipalmitoylphosphatidylcholine vesicle membranes induced by concanavalin A.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 11 ·1975-11-00 ·Pages 4409-13

van der Bosch J, McConnell M

Abstract

The temperature dependence of fatty acid spin label resonance spectra and freeze fracture micrographs of sonicated dipalmitoylphosphatidylcholine vesicles in the absence and presence of concanavalin A demonstrate a strong interaction of concanavalin A with these lipid membranes, which results in fusion of the vesicles. The rate of this reaction as followed with use of magnetic resonance exhibits a pronounced maximum at 36 degrees, the midpoint of the phase transition range of dipalmitoylphosphatidylcholine vesicles. This maximum is discussed in terms of structural fluctuations, which are maximal in the phase transition range of the membranes.

MeSH Terms
Binding Sites Cell Fusion Cell Membrane/metabolism Concanavalin A/metabolism Kinetics Membranes, Artificial Phosphatidylcholines Structure-Activity Relationship Temperature
Chemicals
Membranes, Artificial Phosphatidylcholines Concanavalin A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
van der Bosch J
McConnell M
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33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.11.4409
Published
1975-11-00
Pages
4409-13
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388731
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.11.4409 PMC Full Text View journal details
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