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PMID: 1060132 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Analysis of changes in collagen biosynthesis that occur when chick chondrocytes are grown in 5-bromo-2'-deoxyuridine.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 11 ·1975-11-00 ·Pages 4511-5

Mayne R, Vail MS, Miller EJ

Abstract

Chick embryo chondrocytes, in primary culture, initially synthesize only Type ii collagen (chain composition, [alpha1(II)13), as judged by two criteria: (i) carbosymethyl-cellulose chromatography of the denatured collagen, and (ii) carbosymethyl-cellulose chromatography of the cyanogen bromide peptides derived from the isolated chains. After a period of growth in 5-bromo-2'-deoxyuridine, however, synthesis of two different types of collagen could be detected after differential salt precipitation of the newly synthesized native collagens from neutral salt solutions at 2.2 M NaCl and subsequently at 0.01 M Na2hPO4. By criteria indicated above, the collagen precipitating at 2.2 M NaCl was identified as Type I collagen (chain composition, [alpha(I)]2alpha2), whereas the collagen subsequently precipitated at 0.01 M Na2HPO4 was found to be comprised entirely of alpha1(I) chains, indicating a chain composition, [alpha](I)]3. We propose to designate the latter type of molecule as the Type I trimer.

MeSH Terms
Animals Bromodeoxyuridine/pharmacology Cartilage/cytology,drug effects,metabolism Cells, Cultured Chick Embryo Collagen/analysis,biosynthesis Oligopeptides/analysis Protein Conformation
Chemicals
Oligopeptides Collagen Bromodeoxyuridine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mayne R
Vail M S
Miller E J
References (34)
34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.11.4511
Published
1975-11-00
Pages
4511-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388752
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.11.4511 PMC Full Text View journal details
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