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PMID: 10603417 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

ExoT of cytotoxic Pseudomonas aeruginosa prevents uptake by corneal epithelial cells.

Infection and immunity ·Vol. 68 ·No. 1 ·2000-01-00 ·Pages 403-6

Cowell BA, Chen DY, Frank DW, Vallis AJ, Fleiszig SM

Abstract

The presence of invasion-inhibitory activity that is regulated by the transcriptional activator ExsA of cytotoxic Pseudomonas aeruginosa has previously been proposed. The results of this study show that both ExoT and ExoS, known type III secreted effector proteins of P. aeruginosa that are regulated by ExsA, possess this activity. Invasion was reduced 94.4% by ExoT and 96.0% by ExoS. Invasion-inhibitory activity is not linked to ADP-ribosylation activity, at least for ExoS, since a noncatalytic mutant also inhibits uptake by an epithelial cell line (invasion was reduced 96. 0% by ExoSE381A).

MeSH Terms
Animals Bacterial Proteins/genetics,physiology Cell Line DNA-Binding Proteins/genetics,physiology Epithelium, Corneal/microbiology Genes, Bacterial Mutation Pseudomonas aeruginosa/genetics,pathogenicity Rabbits Trans-Activators/genetics,physiology Virulence/genetics,physiology
Chemicals
Bacterial Proteins DNA-Binding Proteins ExsA protein, bacteria Trans-Activators
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cowell B A
School of Optometry, University of California, Berkeley, California 94720, USA.
Chen D Y
Frank D W
Vallis A J
Fleiszig S M
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.68.1.403-406.2000
Published
2000-01-00
Pages
403-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC97150
Subset
IM
Grants
NEI NIH HHS · R01 EY011221 · United States
NIAID NIH HHS · AI-01289 · United States
NIAID NIH HHS · AI-31665 · United States
NEI NIH HHS · R01-EY11221 · United States
External Links
PubMed source DOI: 10.1128/IAI.68.1.403-406.2000 PMC Full Text View journal details
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