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PMID: 10605091 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Improved sensitivity and coherence selection for [15N,1H]-TROSY elements in triple resonance experiments.

Journal of biomolecular NMR ·Vol. 15 ·No. 2 ·1999-10-00 ·Pages 181-4

Salzmann M, Wider G, Pervushin K, Wüthrich K

Abstract

In experiments with proteins of molecular weights around 100 kDa the implementation of [15N,1H]-TROSY-elements in [15N]-constant-time triple resonance experiments yields sensitivity enhancements of one to two orders of magnitude. An additional gain of 10 to 20% may be obtained with the use of 'sensitivity enhancement elements'. This paper describes a novel sensitivity enhancement scheme which is based on concatenation of the 13C alpha-->15N magnetization transfer with the ST2-PT element, and which enables proper TROSY selection of the 15N multiplet components.

MeSH Terms
Aldehyde-Lyases/chemistry Bacterial Proteins/chemistry Magnetics Nuclear Magnetic Resonance, Biomolecular/methods Sensitivity and Specificity Staphylococcus aureus/enzymology
Chemicals
Bacterial Proteins Aldehyde-Lyases dihydroneopterin aldolase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Salzmann M
Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule Hönggerberg, Zürich, Switzerland.
Wider G
Pervushin K
Wüthrich K
References (8)
8 references, click to expand
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    J Biomol NMR. 1999 Jan;13(1):3-10 PMID: 21080259
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Article Info
Journal
Journal of biomolecular NMR
Abbr.
J Biomol NMR
ISSN
0925-2738
DOI
10.1023/a:1008358030477
Published
1999-10-00
Pages
181-4
Language
English
Region
Netherlands
NLM ID
9110829
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1008358030477 View journal details
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