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PMID: 1061080 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Infectious viral DNA of murine leukemia virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 12 ·1975-12-00 ·Pages 4910-3

Smotkin D, Gianni AM, Rozenblatt S, Weinberg RA

Abstract

A fraction of the unintegrated viral DNA that appears early after infection of mouse cells by Moloney leukemia virus is infectious. The infectivity could be deomonstrated by an XC plaque assay of the cells exposed to DNA co-precipitated with calcium phosphate. The number of plaques deriving from closed-circular, supercoiled DNA was proportional to the concentration of added DNA, indicating that a single DNA molecule of about 5.5 X 10(6) daltons carries all the viral information. Nonsupercoiled viral DNA is also infectious; these molecules appear to be largely double-stranded and 5 to 6 X 10(6) dalthons in mass.

MeSH Terms
Cell Line DNA, Viral/analysis,physiology Kinetics Moloney murine leukemia virus/analysis,pathogenicity Nucleic Acid Conformation Virus Replication
Chemicals
DNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Smotkin D
Gianni A M
Rozenblatt S
Weinberg R A
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.12.4910
Published
1975-12-00
Pages
4910-3
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388842
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.12.4910 PMC Full Text View journal details
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