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PMID: 10611251 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Fibroblast growth factor receptor-mediated rescue of x-ephrin B1-induced cell dissociation in Xenopus embryos.

Molecular and cellular biology ·Vol. 20 ·No. 2 ·2000-01-00 ·Pages 724-34

Chong LD, Park EK, Latimer E, Friesel R, Daar IO

Abstract

The Eph family of receptor tyrosine kinases and their membrane-bound ligands, the ephrins, have been implicated in regulating cell adhesion and migration during development by mediating cell-to-cell signaling events. Genetic evidence suggests that ephrins may transduce signals and become tyrosine phosphorylated during embryogenesis. However, the induction and functional significance of ephrin phosphorylation is not yet clear. Here, we report that when we used ectopically expressed proteins, we found that an activated fibroblast growth factor (FGF) receptor associated with and induced the phosphorylation of ephrin B1 on tyrosine. Moreover, this phosphorylation reduced the ability of overexpressed ephrin B1 to reduce cell adhesion. In addition, we identified a region in the cytoplasmic tail of ephrin B1 that is critical for interaction with the FGF receptor; we also report FGF-induced phosphorylation of ephrins in a neural tissue. This is the first demonstration of communication between the FGF receptor family and the Eph ligand family and implicates cross talk between these two cell surface molecules in regulating cell adhesion.

MeSH Terms
Amino Acid Sequence Animals Binding Sites Cell Adhesion/drug effects Chick Embryo Conserved Sequence Embryo, Nonmammalian/cytology,drug effects,metabolism Ephrin-B1 Fibroblast Growth Factors/pharmacology Gene Expression Membrane Proteins/chemistry,genetics,metabolism Mitogen-Activated Protein Kinases/metabolism Molecular Sequence Data Mutation/genetics Phosphorylation/drug effects Phosphotyrosine/metabolism Protein Binding Receptor Cross-Talk/drug effects Receptors, Fibroblast Growth Factor/genetics,metabolism Receptors, Platelet-Derived Growth Factor/physiology Retina/drug effects,embryology,metabolism Signal Transduction/drug effects Xenopus laevis/embryology
Chemicals
Ephrin-B1 Membrane Proteins Receptors, Fibroblast Growth Factor Phosphotyrosine Fibroblast Growth Factors Receptors, Platelet-Derived Growth Factor Mitogen-Activated Protein Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chong L D
Basic Research Laboratory, National Cancer Institute, Frederick, Maryland 21702, USA.
Park E K
Latimer E
Friesel R
Daar I O
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.20.2.724-734.2000
Published
2000-01-00
Pages
724-34
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC85187
Subset
IM
Grants
NIDCR NIH HHS · R01 DE013248 · United States
NIDCR NIH HHS · DE 13248 · United States
NCI NIH HHS · N01-CO-5600 · United States
External Links
PubMed source DOI: 10.1128/MCB.20.2.724-734.2000 PMC Full Text View journal details
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