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PMID: 10634906 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

ABI3 affects plastid differentiation in dark-grown Arabidopsis seedlings.

The Plant cell ·Vol. 12 ·No. 1 ·2000-01-00 ·Pages 35-52

Rohde A, De Rycke R, Beeckman T, Engler G, Van Montagu M, Boerjan W

Abstract

The Arabidopsis ABSCISIC ACID-INSENSITIVE3 (ABI3) protein has been identified previously as a crucial regulator of late seed development. Here, we show that dark-grown abi3 plants, or abi3 plants returned to the dark after germination in the light, developed and maintained an etioplast with a prominent prolamellar body at developmental stages in which the wild type did not. Overexpression of ABI3 led to the preservation of the plastid ultrastructure that was present at the onset of darkness. These observations suggest that ABI3 plays a role in plastid differentiation pathways in vegetative tissues. Furthermore, the analysis of deetiolated (det1) abi3 double mutants revealed that DET1 and ABI3 impinge on a multitude of common processes. During seed maturation, ABI3 required DET1 to achieve its full expression. Mature det1 abi3 seeds were found to be in a highly germinative state, indicating that germination is controlled by both DET1 and ABI3. During plastid differentiation in leaves of dark-grown plants, DET1 is required for the action of ABI3 as it is during seed development. Together, the results suggest that ABI3 is at least partly regulated by light.

MeSH Terms
Arabidopsis/genetics,growth & development,radiation effects Arabidopsis Proteins Darkness Genes, Plant Germination Intracellular Signaling Peptides and Proteins Mutation Nuclear Proteins/genetics Phenotype Plant Development Plant Proteins/genetics Plants/genetics,radiation effects Plastids/genetics Transcription Factors
Chemicals
ABI3 protein, Arabidopsis Arabidopsis Proteins DET1 protein, Arabidopsis Intracellular Signaling Peptides and Proteins Nuclear Proteins Plant Proteins Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rohde A
Vakgroep Moleculaire Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
De Rycke R
Beeckman T
Engler G
Van Montagu M
Boerjan W
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.12.1.35
Published
2000-01-00
Pages
35-52
Language
English
Region
England
NLM ID
9208688
PMCID
PMC140213
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.12.1.35 PMC Full Text View journal details
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