Characterization of a gamma-carboxyglutamic acid-containing protein from bone.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 5 ·1976-05-00 ·Pages 1447-51

Price PA, Otsuka AA, Poser JW, Kristaponis J, Raman N

Abstract

A gamma-carboxyglutamic acid-containing protein has been purified from the calcified tissues of several vertebrates. The presence of three-gamma-carboxyglutamic acid residues in the bovine protein was established by alkaline hydrolysis and amino acid analysis, a method based upon studies with synthetic gamma-carboxyglutamic acid. The identity of gamma-carboxyglutamic acid in the bovine protein was established by mass spectroscopy on the unknown amino acid isolated from alkaline hydrolysates.

MeSH Terms

Age Factors Amino Acid Sequence Animals Calcification, Physiologic/drug effects Cattle Dentin/analysis Depression, Chemical Female Femur/analysis Fishes Humans Hydroxyapatites/metabolism Lipoproteins/biosynthesis Male Proteins/analysis,isolation & purification,metabolism,pharmacology Tricarboxylic Acids Vitellogenins/biosynthesis,blood

Chemicals

Hydroxyapatites Lipoproteins Proteins Tricarboxylic Acids Vitellogenins

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Price P A

Otsuka A A

Poser J W

Kristaponis J

Raman N

References (13)

13 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.73.5.1447

Published

1976-05-00

Pages

1447-51

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC430313

Subset

External Links

PubMed source DOI: 10.1073/pnas.73.5.1447 PMC Full Text View journal details

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Characterization of a gamma-carboxyglutamic acid-containing protein from bone.

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