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PMID: 1064021 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Biological consequences of incorporation of 5-fluorocytidine in the RNA of 5-fluorouracil-treated eukaryotic cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 5 ·1976-05-00 ·Pages 1528-31

Gleason MK, Fraenkel-Conrat H

Abstract

Treatment of HeLa cells with 5-fluoro-[3H]uracil leads to the incorporation into cellular RNA of 5-fluorocytidine to the extent of about 0.2% of the 5-fluorouridine incorporated. In tobacco mosaic virus RNA produced in tobacco leaves this ratio is one order of magnitude lower. Copolymers of cytidylic with 5-fluorocytidylic acids show unchanged template activity with E. coli RNA polymerase, but slightly altered messenger activity in the wheat germ system, compared to poly(C), and it is suggested that some of the biological consequences of 5-fluorouracil treatment of living cells and organisms may be attributed to this mechanism.

MeSH Terms
Cytosine/analogs & derivatives Flucytosine/analogs & derivatives,analysis,metabolism Fluorouracil/metabolism HeLa Cells Mutation RNA, Messenger/metabolism RNA, Viral/metabolism Structure-Activity Relationship Templates, Genetic Tobacco Mosaic Virus
Chemicals
RNA, Messenger RNA, Viral Cytosine Flucytosine Fluorouracil
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gleason M K
Fraenkel-Conrat H
References (21)
21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.5.1528
Published
1976-05-00
Pages
1528-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430330
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.5.1528 PMC Full Text View journal details
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