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PMID: 1064024 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

In vitro transcription of chromatin in the presence of a mercurated nucleotide.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 5 ·1976-05-00 ·Pages 1564-7

Crouse GF, Fodor JB, Doty P

Abstract

Mercurated uridine triphosphate has been used to label transcripts of chicken reticulocyte chromatin made with Escherichia coli RNA polymerase. The mercury-labeled RNA product can be completely separated from endogenous RNA sequences in the chromatin by passage through a sulfhydryl Sepharose column. Globin cDNA hybridization to the transcript shows that only 2.6 x 10-5 of the transcript is globin RNA. In contrast to this result, erythrocte chromatin transcript contains less than one tenth as many globin RNA sequences.

MeSH Terms
Animals Chickens Chromatin/metabolism Chromatography, Affinity DNA Erythrocytes/metabolism Globins Male Mercury Nucleic Acid Hybridization RNA, Messenger/biosynthesis,isolation & purification Reticulocytes/metabolism Transcription, Genetic Uracil Nucleotides
Chemicals
Chromatin RNA, Messenger Uracil Nucleotides Globins DNA Mercury
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Crouse G F
Fodor J B
Doty P
References (16)
16 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.5.1564
Published
1976-05-00
Pages
1564-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430338
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.5.1564 PMC Full Text View journal details
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