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PMID: 1064875 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Mapping of adenovirus messenger RNA by electron microscopy.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 6 ·1976-06-00 ·Pages 2069-71

Westphal H, Meyer J, Maizel JV

Abstract

Late adenovirus messenger RNA was annealed to complementary regions of partially melted viral double-stranded DNA. The RNA. DNA hybrid regions within the DNA molecules were visualized as loops in the electron microscope. Loops occurred at several regions of the DNA, most frequently, however, at a location near the center of the molecule. This hybridization technique appears well suited for an accurate mapping of messenger RNA, as well as for studies of RNA processing.

MeSH Terms

Adenoviridae Chromosome Mapping DNA, Viral Microscopy, Electron Nucleic Acid Hybridization Polyribosomes RNA, Messenger RNA, Viral

Chemicals

DNA, Viral RNA, Messenger RNA, Viral

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Westphal H

Meyer J

Maizel J V

References (14)

14 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.73.6.2069

Published

1976-06-00

Pages

2069-71

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC430450

Subset

External Links

PubMed source DOI: 10.1073/pnas.73.6.2069 PMC Full Text View journal details

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Mapping of adenovirus messenger RNA by electron microscopy.

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